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PDBsum entry 2jmc
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Signaling protein
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PDB id
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2jmc
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Contents |
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* Residue conservation analysis
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PDB id:
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Signaling protein
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Title:
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Chimer between spc-sh3 and p41
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Structure:
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Spectrin alpha chain, brain and p41 peptide chimera. Chain: a. Synonym: spectrin, non-erythroid alpha chain, fodrin alpha chain. Engineered: yes
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Source:
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Gallus gallus. Chicken. Organism_taxid: 9031. Gene: sptan1, spta2. Expressed in: escherichia coli. Expression_system_taxid: 562.
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NMR struc:
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20 models
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Authors:
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N.A.J.Van Nuland,A.M.Candel,J.C.Martinez,F.Conejero-Lara,M.Bruix
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Key ref:
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A.M.Candel
et al.
(2007).
The high-resolution NMR structure of a single-chain chimeric protein mimicking a SH3-peptide complex.
Febs Lett,
581,
687-692.
PubMed id:
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Date:
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02-Nov-06
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Release date:
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24-Apr-07
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PROCHECK
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Headers
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References
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P07751
(SPTN1_CHICK) -
Spectrin alpha chain, non-erythrocytic 1 from Gallus gallus
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Seq:
Struc:
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2477 a.a.
77 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 33 residue positions (black
crosses)
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Febs Lett
581:687-692
(2007)
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PubMed id:
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The high-resolution NMR structure of a single-chain chimeric protein mimicking a SH3-peptide complex.
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A.M.Candel,
F.Conejero-Lara,
J.C.Martinez,
N.A.van Nuland,
M.Bruix.
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ABSTRACT
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Here we present the high-resolution NMR structure of a chimera (SPCp41) between
alpha-spectrin SH3 domain and the decapeptide p41. The tertiary structure mimics
perfectly the interactions typically found in SH3-peptide complexes and is
remarkably similar to that of the complex between the separate Spc-SH3 domain
and ligand p41. Relaxation data confirm the tight binding between the ligand and
SH3 part of the chimera. This chimera will serve as a tool for a deeper
understanding of the relationship between structure and thermodynamics of
binding using a combination of NMR, stability and site-directed mutagenesis
studies, which can lead to an effective strategy for ligand design.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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M.Guttman,
J.H.Prieto,
T.M.Handel,
P.J.Domaille,
and
E.A.Komives
(2010).
Structure of the minimal interface between ApoE and LRP.
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J Mol Biol,
398,
306-319.
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PDB codes:
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A.M.Candel,
E.S.Cobos,
F.Conejero-Lara,
and
J.C.Martinez
(2009).
Evaluation of folding co-operativity of a chimeric protein based on the molecular recognition between polyproline ligands and SH3 domains.
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Protein Eng Des Sel,
22,
597-606.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
codes are
shown on the right.
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Links
