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UniProt functional annotation for Q97ZE2 | ||
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| UniProt code: Q97ZE2. |
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| Organism: | |
Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) (Sulfolobus solfataricus). |
| Taxonomy: | |
Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Saccharolobus. |
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| Function: | |
Catalyzes the reversible phosphorylation of UMP to UDP, with ATP as the most efficient phosphate donor. Is also able to phosphorylate dUMP, although much less efficiently. {ECO:0000269|PubMed:17297917}. |
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| Catalytic activity: | |
Reaction=ATP + UMP = ADP + UDP; Xref=Rhea:RHEA:24400, ChEBI:CHEBI:30616, ChEBI:CHEBI:57865, ChEBI:CHEBI:58223, ChEBI:CHEBI:456216; EC=2.7.4.22; |
| Activity regulation: | |
Unlike most bacteria, is not activated by GTP. UTP acts as a competitive inhibitor against both substrates. High concentration of UMP abolishes the inhibition of UTP at low ATP concentrations, indicating that UTP binds to the acceptor site (UMP site). {ECO:0000269|PubMed:17297917}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=14 uM for UMP (at pH 7.2) {ECO:0000269|PubMed:17297917}; KM=81 uM for ATP (at pH 7.2) {ECO:0000269|PubMed:17297917}; Vmax=5 umol/min/mg enzyme (at pH 5.5) {ECO:0000269|PubMed:17297917}; Vmax=45 umol/min/mg enzyme (at pH 7.2) {ECO:0000269|PubMed:17297917}; Vmax=12 umol/min/mg enzyme (at pH 8.5) {ECO:0000269|PubMed:17297917}; pH dependence: Optimum pH is 7.0. {ECO:0000269|PubMed:17297917}; |
| Pathway: | |
Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1. |
| Subunit: | |
Homohexamer. {ECO:0000269|PubMed:17297917}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250}. |
| Miscellaneous: | |
Catalysis proceeds by a sequential bi-bi reaction mechanism of random order. |
| Similarity: | |
Belongs to the UMP kinase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.