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PDBsum entry 2j12
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protein metals Protein-protein interface(s) links
Viral protein/receptor PDB id
2j12

 

 

 

 

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Contents
Protein chains
182 a.a. *
120 a.a. *
Metals
_CA
Waters ×287
* Residue conservation analysis
PDB id:
2j12
Name: Viral protein/receptor
Title: Ad37 fibre head in complex with car d1
Structure: Fiber protein. Chain: a. Fragment: fibre head, residues 177-365. Synonym: adenovirus 37 fibre head. Engineered: yes. Coxsackievirus and adenovirus receptor. Chain: b. Fragment: domain d1, residues 15-140. Synonym: coxsackievirus b-adenovirus receptor, hcar, cvb3-binding
Source: Human adenovirus d37. Organism_taxid: 52275. Strain: type 37. Expressed in: escherichia coli. Expression_system_taxid: 511693. Homo sapiens. Human. Organism_taxid: 9606. Expression_system_taxid: 562.
Biol. unit: Hexamer (from PDB file)
Resolution:
1.50Å     R-factor:   0.149     R-free:   0.169
Authors: E.Seiradake,H.Lortat-Jacob,O.Billet,E.J.Kremer,S.Cusack
Key ref:
E.Seiradake et al. (2006). Structural and mutational analysis of human Ad37 and canine adenovirus 2 fiber heads in complex with the D1 domain of coxsackie and adenovirus receptor. J Biol Chem, 281, 33704-33716. PubMed id: 16923808 DOI: 10.1074/jbc.M605316200
Date:
08-Aug-06     Release date:   29-Aug-06    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q80S15  (Q80S15_9ADEN) -  Fiber protein (Fragment) from Human adenovirus D37
Seq:
Struc: 		210 a.a.
182 a.a.
Protein chain
Pfam   ArchSchema ?
P78310  (CXAR_HUMAN) -  Coxsackievirus and adenovirus receptor from Homo sapiens
Seq:
Struc: 		365 a.a.
120 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 

 
DOI no: 10.1074/jbc.M605316200 J Biol Chem 281:33704-33716 (2006)
PubMed id: 16923808  
 
 
Structural and mutational analysis of human Ad37 and canine adenovirus 2 fiber heads in complex with the D1 domain of coxsackie and adenovirus receptor.
E.Seiradake, H.Lortat-Jacob, O.Billet, E.J.Kremer, S.Cusack.
 
  ABSTRACT  
 
Adenovirus fibers from most serotypes bind the D1 domain of coxsackie and adenovirus receptor (CAR), although the binding residues are not strictly conserved. To understand this further, we determined the crystal structures of canine adenovirus serotype 2 (CAV-2) and the human adenovirus serotype 37 (HAd37) in complex with human CAR D1 at 2.3 and 1.5A resolution, respectively. Structure comparison with the HAd12 fiber head-CAR D1 complex showed that the overall topology of the interaction is conserved but that the interfaces differ in number and identity of interacting residues, shape complementarity, and degree of conformational adaptation. Using surface plasmon resonance, we characterized the binding affinity to CAR D1 of wild type and mutant CAV-2 and HAd37 fiber heads. We found that CAV-2 has the highest affinity but fewest direct interactions, with the reverse being true for HAd37. Moreover, we found that conserved interactions can have a minor contribution, whereas serotype-specific interactions can be essential. These results are discussed in the light of virus evolution and design of adenovirus vectors for gene transfer.
 
  Selected figure(s)  
 
Figure 2.
FIGURE 2. Cartoon representations of adenovirus fiber heads in complex with CAR D1. HAd12, HAd37, and CAV-2 fiber heads are colored blue, pink, and yellow, respectively. CAR D1 is in green. A, top view of the structure of HAd12 fiber head in complex with CAR D1 (13). B, as A, but HAd37 fiber head. C, as A and B, but CAV-2 fiber head. D, part of the binding interface between HAd12 fiber head and CAR D1 showing some key interacting residues. E, equivalent region of interface between HAd37 fiber head and CAR D1 showing conservation of the interactions and additional unique interactions provided by HAd37 Glu^351 from the IJ loop. F, same region of the CAV-2 fiber head and CAR D1 interface showing equivalent interactions made by sometimes different fiber residues. Hydrogen bonds are shown in dotted lines. Residues marked with an asterisk are not fully represented for clarity. 		Figure 6.
FIGURE 6. SPR sensorgrams of wild type and mutant adenovirus fiber heads binding to immobilized CAR D1. A, wild type HAd37 fiber head; B, HAd37 fiber head mutant S299A; C, HAd37 fiber head mutant E351A; D, wild type CAV-2 fiber head; E, CAV-2 fiber head mutant T441A; F, CAV-2 fiber head mutant R384E; G, CAV-2 fiber head mutant R515A; H, CAV-2 fiber head mutant G370D; I, CAV-2 fiber head mutant R384A. , results calculated from the sensorgrams. The S.E. did not exceed 15% of the K[d]. , Lortat-Jacob et al. (51) using the same experimental set-up and CAR construct as in this work. , Kirby et al. (67) using a longer CAR construct.
 
  The above figures are reprinted by permission from the ASBMB: J Biol Chem (2006, 281, 33704-33716) copyright 2006.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
21366418 D.Henaff, S.Salinas, and E.J.Kremer (2011).
An adenovirus traffic update: from receptor engagement to the nuclear pore.
  Future Microbiol, 6, 179-192.  
20071571 K.Cupelli, S.Müller, B.D.Persson, M.Jost, N.Arnberg, and T.Stehle (2010).
Structure of adenovirus type 21 knob in complex with CD46 reveals key differences in receptor contacts among species B adenoviruses.
  J Virol, 84, 3189-3200.
PDB codes: 3l88 3l89
19498462 E.Seiradake, A.C.von Philipsborn, M.Henry, M.Fritz, H.Lortat-Jacob, M.Jamin, W.Hemrika, M.Bastmeyer, S.Cusack, and A.A.McCarthy (2009).
Structure and functional relevance of the Slit2 homodimerization domain.
  EMBO Rep, 10, 736-741.
PDB code: 2wfh
19119424 E.Seiradake, D.Henaff, H.Wodrich, O.Billet, M.Perreau, C.Hippert, F.Mennechet, G.Schoehn, H.Lortat-Jacob, H.Dreja, S.Ibanes, V.Kalatzis, J.P.Wang, R.W.Finberg, S.Cusack, and E.J.Kremer (2009).
The cell adhesion molecule "CAR" and sialic acid on human erythrocytes influence adenovirus in vivo biodistribution.
  PLoS Pathog, 5, e1000277.
PDB codes: 2w9l 2wbv 2wbw
20041185 J.Rebetz, M.Na, C.Su, B.Holmqvist, A.Edqvist, C.Nyberg, B.Widegren, L.G.Salford, H.O.Sjögren, N.Arnberg, Q.Qian, and X.Fan (2009).
Fiber mediated receptor masking in non-infected bystander cells restricts adenovirus cell killing effect but promotes adenovirus host co-existence.
  PLoS One, 4, e8484.  
19461877 S.Salinas, L.G.Bilsland, D.Henaff, A.E.Weston, A.Keriel, G.Schiavo, and E.J.Kremer (2009).
CAR-associated vesicular transport of an adenovirus in motor neuron axons.
  PLoS Pathog, 5, e1000442.  
19342221 T.Stehle, and J.M.Casasnovas (2009).
Specificity switching in virus-receptor complexes.
  Curr Opin Struct Biol, 19, 181-188.  
18657508 D.Hatherley, S.C.Graham, J.Turner, K.Harlos, D.I.Stuart, and A.N.Barclay (2008).
Paired receptor specificity explained by structures of signal regulatory proteins alone and complexed with CD47.
  Mol Cell, 31, 266-277.
PDB codes: 2jjs 2jjt 2jju 2jjv 2jjw 2vsc
18216088 G.Schoehn, M.El Bakkouri, C.M.Fabry, O.Billet, L.F.Estrozi, L.Le, D.T.Curiel, A.V.Kajava, R.W.Ruigrok, and E.J.Kremer (2008).
Three-dimensional structure of canine adenovirus serotype 2 capsid.
  J Virol, 82, 3192-3203.  
18424543 K.Sirikanchana, J.L.Shisler, and B.J.Mariñas (2008).
Effect of exposure to UV-C irradiation and monochloramine on adenovirus serotype 2 early protein expression and DNA replication.
  Appl Environ Microbiol, 74, 3774-3782.  
18354474 M.A.Shannon, P.W.Bohn, M.Elimelech, J.G.Georgiadis, B.J.Mariñas, and A.M.Mayes (2008).
Science and technology for water purification in the coming decades.
  Nature, 452, 301-310.  
17376928 K.J.Excoffon, N.Gansemer, G.Traver, and J.Zabner (2007).
Functional effects of coxsackievirus and adenovirus receptor glycosylation on homophilic adhesion and adenoviral infection.
  J Virol, 81, 5573-5578.  
17229706 M.Perreau, F.Mennechet, N.Serratrice, J.N.Glasgow, D.T.Curiel, H.Wodrich, and E.J.Kremer (2007).
Contrasting effects of human, canine, and hybrid adenovirus vectors on the phenotypical and functional maturation of human dendritic cells: implications for clinical efficacy.
  J Virol, 81, 3272-3284.  
17712332 M.Perreau, M.C.Guérin, C.Drouet, and E.J.Kremer (2007).
Interactions between human plasma components and a xenogenic adenovirus vector: reduced immunogenicity during gene transfer.
  Mol Ther, 15, 1998-2007.  
17913818 R.J.Ossiboff, and J.S.Parker (2007).
Identification of regions and residues in feline junctional adhesion molecule required for feline calicivirus binding and infection.
  J Virol, 81, 13608-13621.  
18074396 R.L.Rich, and D.G.Myszka (2007).
Survey of the year 2006 commercial optical biosensor literature.
  J Mol Recognit, 20, 300-366.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.

 

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