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PDBsum entry 2j06
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Signal transduction
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PDB id
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2j06
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Signal transduction
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Title:
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Crystal structure of the rasgap sh3 domain at 1.8 angstrom resolution
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Structure:
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Ras gtpase-activating protein 1. Chain: a, b. Fragment: sh3 domain, residues 281-341. Synonym: gtpase-activating protein, gap, ras p21 protein activator, p120gap, rasgap. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 469008.
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Resolution:
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1.80Å
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R-factor:
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0.208
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R-free:
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0.240
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Authors:
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B.Ross,M.Gajhede,O.Kristensen
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Key ref:
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B.Ross
et al.
(2007).
High resolution crystal structures of the p120 RasGAP SH3 domain.
Biochem Biophys Res Commun,
353,
463-468.
PubMed id:
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Date:
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01-Aug-06
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Release date:
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02-Jan-07
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PROCHECK
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Headers
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References
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P20936
(RASA1_HUMAN) -
Ras GTPase-activating protein 1 from Homo sapiens
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Seq:
Struc:
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1047 a.a.
62 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 1 residue position (black
cross)
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Biochem Biophys Res Commun
353:463-468
(2007)
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PubMed id:
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High resolution crystal structures of the p120 RasGAP SH3 domain.
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B.Ross,
O.Kristensen,
D.Favre,
J.Walicki,
J.S.Kastrup,
C.Widmann,
M.Gajhede.
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ABSTRACT
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X-ray structures of two crystal forms of the Src homology 3 domain (SH3) of the
Ras GTPase activating protein (RasGAP) were determined at 1.5 and 1.8A
resolution. The overall structure comprises a single domain with two tightly
packed beta-sheets linked by a short helical segment. An important motif for
peptide binding in other SH3 domains is not conserved in RasGAP. The RasGAP SH3
domain forms dimers in the crystal structures, which may provide new functional
insight. The dimer interface involves residues also present in a peptide
previously identified as an apoptotic sensitizer of tumor cells.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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X.Y.Yang,
M.Guan,
D.Vigil,
C.J.Der,
D.R.Lowy,
and
N.C.Popescu
(2009).
p120Ras-GAP binds the DLC1 Rho-GAP tumor suppressor protein and inhibits its RhoA GTPase and growth-suppressing activities.
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Oncogene,
28,
1401-1409.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
