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UniProt functional annotation for Q92PC8 | ||
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| UniProt code: Q92PC8. |
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| Organism: | |
Rhizobium meliloti (strain 1021) (Ensifer meliloti) (Sinorhizobium meliloti). |
| Taxonomy: | |
Bacteria; Proteobacteria; Alphaproteobacteria; Rhizobiales; Rhizobiaceae; Sinorhizobium/Ensifer group; Sinorhizobium. |
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| Function: | |
Involved in the biosynthesis of cobalamin (vitamin B12). Catalyzes the oxidative fragmentation and contraction of the isoalloxazine heterocycle and the cleavage of the ribityl tail of FMN(2) to form 5,6-dimethylbenzimidazole (DMB) and D-erythrose 4- phosphate (E4P). NAD(P)H is only required initially to reduce FMN and oxygen drives the oxidative fragmentation. {ECO:0000269|PubMed:16537439, ECO:0000269|PubMed:17377583}. |
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| Catalytic activity: | |
Reaction=FMNH2 + O2 = 5,6-dimethylbenzimidazole + D-erythrose 4- phosphate + dialurate + H(+); Xref=Rhea:RHEA:27345, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15890, ChEBI:CHEBI:16897, ChEBI:CHEBI:57618, ChEBI:CHEBI:140629; EC=1.13.11.79; Evidence={ECO:0000269|PubMed:17377583}; |
| Activity regulation: | |
Inhibited by high concentrations of FMN. {ECO:0000269|PubMed:17377583}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=64 uM for FMN {ECO:0000269|PubMed:17377583}; KM=4.4 mM for NADP {ECO:0000269|PubMed:17377583}; KM=5.1 mM for NAD {ECO:0000269|PubMed:17377583}; Note=Kcat is 15 (h-1), 7.2 (h-1) and 7.6 (h-1) for FMN, NAD and NADP, respectively.; |
| Subunit: | |
Homooctamer. {ECO:0000269|PubMed:17377583}. |
| Disruption phenotype: | |
Cells lacking this gene are unable to grow in minimal media and fails to establish a symbiosis with alfalfa, and these defects can be rescued by the addition of vitamin B12 (cyanocobalamin) or the lower ligand of cobalamin, 5,6- dimethylbenzimidazole (DMB). {ECO:0000269|PubMed:16537439}. |
| Similarity: | |
Belongs to the BluB family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.