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PDBsum entry 2if5
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Transcription
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PDB id
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2if5
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Contents |
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* Residue conservation analysis
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PDB id:
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| Name: |
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Transcription
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Title:
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Structure of the poz domain of human lrf, a master regulator of oncogenesis
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Structure:
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Zinc finger and btb domain-containing protein 7a. Chain: a. Fragment: btb/poz-domain, residues 9-128. Synonym: leukemia/lymphoma- related factor, factor that binds to inducer of short transcripts protein 1, factor binding ist protein 1, fbi-1, HIV-1 1st-binding protein 1, ttf-i-interacting peptide 21, tip21. Engineered: yes
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Source:
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Homo sapiens. Human. Organism_taxid: 9606. Gene: zbtb7a, fbi1, lrf, zbtb7. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
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Biol. unit:
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Dimer (from PDB file)
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Resolution:
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2.00Å
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R-factor:
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0.232
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R-free:
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0.291
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Authors:
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F.D.Schubot,D.S.Waugh,J.Tropea
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Key ref:
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F.D.Schubot
et al.
(2006).
Structure of the POZ domain of human LRF, a master regulator of oncogenesis.
Biochem Biophys Res Commun,
351,
1-6.
PubMed id:
DOI:
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Date:
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20-Sep-06
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Release date:
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21-Nov-06
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PROCHECK
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Headers
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References
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O95365
(ZBT7A_HUMAN) -
Zinc finger and BTB domain-containing protein 7A from Homo sapiens
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Seq:
Struc:
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584 a.a.
115 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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DOI no:
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Biochem Biophys Res Commun
351:1-6
(2006)
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PubMed id:
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Structure of the POZ domain of human LRF, a master regulator of oncogenesis.
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F.D.Schubot,
J.E.Tropea,
D.S.Waugh.
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ABSTRACT
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The proto-oncogenic properties of the POK family of transcriptional repressors
BCL6, PLZF, and LRF have been well established. These proteins utilize their
amino-terminal POZ domains for multimerization and the recruitment of
co-repressors. Because LRF represses the production of the tumor suppressor
p19(Arf) (ARF), it is regarded as an attractive therapeutic target for the
treatment of many types of cancer. The crystal structure of the LRF POZ domain
reveals a high degree of structural conservation with the corresponding domains
of BCL6 and PLZF. However, striking differences between the electrostatic
properties of the BCL6 and LRF POZ domains suggest that if, like BCL6, LRF
interacts with the co-repressor SMRT, it almost certainly uses a different
mechanism to do so. These differences may also explain why LRF interacts with
BCL6 but not with PLZF. Finally, the conservation of crystal packing contacts
suggests the probable location of the interface that mediates LRF/BCL6 complex
formation.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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H.Qu,
D.Qu,
F.Chen,
Z.Zhang,
B.Liu,
and
H.Liu
(2010).
ZBTB7 overexpression contributes to malignancy in breast cancer.
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Cancer Invest,
28,
672-678.
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L.Jiang,
M.K.Siu,
O.G.Wong,
K.F.Tam,
E.W.Lam,
H.Y.Ngan,
X.F.Le,
E.S.Wong,
H.Y.Chan,
and
A.N.Cheung
(2010).
Overexpression of proto-oncogene FBI-1 activates membrane type 1-matrix metalloproteinase in association with adverse outcome in ovarian cancers.
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Mol Cancer,
9,
318.
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S.Fourquet,
R.Guerois,
D.Biard,
and
M.B.Toledano
(2010).
Activation of NRF2 by nitrosative agents and H2O2 involves KEAP1 disulfide formation.
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J Biol Chem,
285,
8463-8471.
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N.Ito,
M.Watanabe-Matsui,
K.Igarashi,
and
K.Murayama
(2009).
Crystal structure of the Bach1 BTB domain and its regulation of homodimerization.
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Genes Cells,
14,
167-178.
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M.A.Stead,
G.O.Rosbrook,
J.M.Hadden,
C.H.Trinh,
S.B.Carr,
and
S.C.Wright
(2008).
Structure of the wild-type human BCL6 POZ domain.
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Acta Crystallogr Sect F Struct Biol Cryst Commun,
64,
1101-1104.
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PDB code:
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M.Laudes,
R.Bilkovski,
F.Oberhauser,
A.Droste,
M.Gomolka,
U.Leeser,
M.Udelhoven,
and
W.Krone
(2008).
Transcription factor FBI-1 acts as a dual regulator in adipogenesis by coordinated regulation of cyclin-A and E2F-4.
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J Mol Med,
86,
597-608.
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K.Apostolopoulou,
I.S.Pateras,
K.Evangelou,
P.K.Tsantoulis,
M.Liontos,
C.Kittas,
D.G.Tiniakos,
A.Kotsinas,
C.Cordon-Cardo,
and
V.G.Gorgoulis
(2007).
Gene amplification is a relatively frequent event leading to ZBTB7A (Pokemon) overexpression in non-small cell lung cancer.
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J Pathol,
213,
294-302.
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P.J.Stogios,
L.Chen,
and
G.G.Privé
(2007).
Crystal structure of the BTB domain from the LRF/ZBTB7 transcriptional regulator.
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Protein Sci,
16,
336-342.
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PDB code:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
