UniProt functional annotation for P34021



	UniProt functional annotation for P34021

UniProt code: P34021.

Organism: Drosophila melanogaster (Fruit fly).

Taxonomy: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.

Function: Receptor for ecdysone (PubMed:1913820, PubMed:30293839). Binds to ecdysone response elements (ECRES) following ecdysone-binding, and recruitment of a complex containing the histone methyltransferase trr, leads to activate transcription of target genes (PubMed:1913820, PubMed:30293839). {ECO:0000269|PubMed:1913820, ECO:0000269|PubMed:30293839}.

Subunit: Heterodimer of USP and ECR (PubMed:8247157). Only the heterodimer is capable of high-affinity binding to ecdysone (PubMed:8247157). Interacts with trr in an ecdysone-dependent manner (PubMed:14603321). Upon ecdysone stimulation, interacts with Nup98 (PubMed:28366641). {ECO:0000269|PubMed:14603321, ECO:0000269|PubMed:28366641, ECO:0000269|PubMed:8247157}.

Subcellular location: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00407, ECO:0000269|PubMed:1913820, ECO:0000269|PubMed:30293839}.

Tissue specificity: Isoform B1 predominates over isoform A in larval tissues, imaginal histoblast nests and midgut islands. Isoform A predominates over B1 in imaginal disks, and the larval prothoracic gland. {ECO:0000269|PubMed:1913820}.

Developmental stage: In the salivary glands of mid instar larvae levels increase during puff stage 1 at 86-94 hours of development then remain relatively constant until the premetamorphic pulse of ecdysone in late larvae. Levels diminish dramatically from puff stage 7 onwards. Levels increase in the prepupal period during puff stage 13-14, the level remains stable until stage 21. A decrease in levels at puff stage 7 is also seen in the Malpighian tubules and less dramatically in the fat body and gut. In the wing disk the relatively low level remains unchanged. {ECO:0000269|PubMed:1913820, ECO:0000269|PubMed:8223281}.

Disruption phenotype: RNAi-mediated knockdown blocks ecdysone-dependent fat body cell migration into the pupal head. {ECO:0000269|PubMed:30293839}.

Similarity: Belongs to the nuclear hormone receptor family. NR1 subfamily. {ECO:0000305}.

Sequence caution: Sequence=AAL13804.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Drosophila melanogaster (Fruit fly).
Taxonomy:		Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora.



Function:		Receptor for ecdysone (PubMed:1913820, PubMed:30293839). Binds to ecdysone response elements (ECRES) following ecdysone-binding, and recruitment of a complex containing the histone methyltransferase trr, leads to activate transcription of target genes (PubMed:1913820, PubMed:30293839). {ECO:0000269\|PubMed:1913820, ECO:0000269\|PubMed:30293839}.


Subunit:		Heterodimer of USP and ECR (PubMed:8247157). Only the heterodimer is capable of high-affinity binding to ecdysone (PubMed:8247157). Interacts with trr in an ecdysone-dependent manner (PubMed:14603321). Upon ecdysone stimulation, interacts with Nup98 (PubMed:28366641). {ECO:0000269\|PubMed:14603321, ECO:0000269\|PubMed:28366641, ECO:0000269\|PubMed:8247157}.
Subcellular location:		Nucleus {ECO:0000255\|PROSITE-ProRule:PRU00407, ECO:0000269\|PubMed:1913820, ECO:0000269\|PubMed:30293839}.
Tissue specificity:		Isoform B1 predominates over isoform A in larval tissues, imaginal histoblast nests and midgut islands. Isoform A predominates over B1 in imaginal disks, and the larval prothoracic gland. {ECO:0000269\|PubMed:1913820}.
Developmental stage:		In the salivary glands of mid instar larvae levels increase during puff stage 1 at 86-94 hours of development then remain relatively constant until the premetamorphic pulse of ecdysone in late larvae. Levels diminish dramatically from puff stage 7 onwards. Levels increase in the prepupal period during puff stage 13-14, the level remains stable until stage 21. A decrease in levels at puff stage 7 is also seen in the Malpighian tubules and less dramatically in the fat body and gut. In the wing disk the relatively low level remains unchanged. {ECO:0000269\|PubMed:1913820, ECO:0000269\|PubMed:8223281}.
Disruption phenotype:		RNAi-mediated knockdown blocks ecdysone-dependent fat body cell migration into the pupal head. {ECO:0000269\|PubMed:30293839}.
Similarity:		Belongs to the nuclear hormone receptor family. NR1 subfamily. {ECO:0000305}.
Sequence caution:		Sequence=AAL13804.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};