PDBsum entry 2han

Transcription/DNA

PDB id: 2han

Contents

Protein chains

78 a.a. *

87 a.a. *

DNA/RNA

Metals

_ZN ×4

Waters ×222

* Residue conservation analysis

PDB id:

2han

Name:

Transcription/DNA

Title:

Structural basis of heterodimeric ecdysteroid receptor interaction with natural response element hsp27 gene promoter

Structure:

5'-d( Cp Ap Ap Gp Gp Gp Tp Tp Cp Ap Ap Tp Gp Cp Ap Cp Tp Tp Gp T)-3'. Chain: c. Engineered: yes. Other_details: natural ecdysone response element. 5'-d( Gp Ap Cp Ap Ap Gp Tp Gp Cp Ap Tp Tp Gp Ap Ap Cp Cp Cp Tp T)-3'. Chain: d. Engineered: yes.

Source:

Synthetic: yes. Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Gene: usp, cf1, nr2b4. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: ecr, nr1h1.

Resolution:

1.95Å R-factor: 0.182 R-free: 0.217

Authors:

M.Jakob,R.Kolodziejczyk,M.Orlowski,S.Krzywda,A.Kowalska,J.Dutko- Gwozdz,T.Gwozdz,M.Kochman,M.Jaskolski,A.Ozyhar

Key ref:

M.Jakób et al. (2007). Novel DNA-binding element within the C-terminal extension of the nuclear receptor DNA-binding domain. Nucleic Acids Res, 35, 2705-2718. PubMed id: 17426125

Date:

13-Jun-06 Release date: 22-May-07

Protein chain

P20153  (USP_DROME) -  Protein ultraspiracle from Drosophila melanogaster

Seq: 508 a.a.

Struc: 78 a.a.

Protein chain

P34021  (ECR_DROME) -  Ecdysone receptor from Drosophila melanogaster

Seq: 878 a.a.

Struc: 87 a.a.

DNA/RNA chains

C-A-A-G-G-G-T-T-C-A-A-T-G-C-A-C-T-T-G-T 20 bases

G-A-C-A-A-G-T-G-C-A-T-T-G-A-A-C-C-C-T-T 20 bases

Nucleic Acids Res 35:2705-2718 (2007)

PubMed id: 17426125

Novel DNA-binding element within the C-terminal extension of the nuclear receptor DNA-binding domain.

M.Jakób, R.Kołodziejczyk, M.Orłowski, S.Krzywda, A.Kowalska, J.Dutko-Gwóźdź, T.Gwóźdź, M.Kochman, M.Jaskólski, A.Ozyhar.

ABSTRACT

The heterodimer of the ecdysone receptor (EcR) and ultraspiracle (Usp), members of the nuclear receptors superfamily, is considered as the functional receptor for ecdysteroids initiating molting and metamorphosis in insects. Here we report the 1.95 A structure of the complex formed by the DNA-binding domains (DBDs) the EcR and the Usp, bound to the natural pseudopalindromic response element. Comparison of the structure with that obtained previously, using an idealized response element, shows how the EcRDBD, which has been previously reported to possess extraordinary flexibility, accommodates DNA-induced structural changes. Part of the C-terminal extension (CTE) of the EcRDBD folds into an alpha-helix whose location in the minor groove does not match any of the locations previously observed for nuclear receptors. Mutational analyses suggest that the alpha-helix is a component of EcR-box, a novel element indispensable for DNA-binding and located within the nuclear receptor CTE. This element seems to be a general feature of all known EcRs.