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PDBsum entry 2gri
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Viral protein
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PDB id
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2gri
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References listed in PDB file
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Key reference
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Title
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Nuclear magnetic resonance structure of the n-Terminal domain of nonstructural protein 3 from the severe acute respiratory syndrome coronavirus.
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Authors
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P.Serrano,
M.A.Johnson,
M.S.Almeida,
R.Horst,
T.Herrmann,
J.S.Joseph,
B.W.Neuman,
V.Subramanian,
K.S.Saikatendu,
M.J.Buchmeier,
R.C.Stevens,
P.Kuhn,
K.Wüthrich.
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Ref.
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J Virol, 2007,
81,
12049-12060.
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above have
been manually determined.
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Abstract
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This paper describes the structure determination of nsp3a, the N-terminal domain
of the severe acute respiratory syndrome coronavirus (SARS-CoV) nonstructural
protein 3. nsp3a exhibits a ubiquitin-like globular fold of residues 1 to 112
and a flexibly extended glutamic acid-rich domain of residues 113 to 183. In
addition to the four beta-strands and two alpha-helices that are common to
ubiquitin-like folds, the globular domain of nsp3a contains two short helices
representing a feature that has not previously been observed in these proteins.
Nuclear magnetic resonance chemical shift perturbations showed that these unique
structural elements are involved in interactions with single-stranded RNA.
Structural similarities with proteins involved in various cell-signaling
pathways indicate possible roles of nsp3a in viral infection and persistence.
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