PDBsum entry 2gee

Protein binding, oncoprotein

PDB id

2gee

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Contents

			Protein chain

					188 a.a. *

			Waters ×384

* Residue conservation analysis

PDB id:

2gee

Links

Name:

Protein binding, oncoprotein

Title:

Crystal structure of human type iii fibronectin extradomain b and domain 8

Structure:

Hypothetical protein. Chain: a. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: dkfzp686o1166. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.01Å

R-factor:

0.249

R-free:

0.301

Authors:

S.Bencharit,C.B.Cui,A.Siddiqui,E.L.Howard-Williams,I.Aukhil

Key ref:

S.Bencharit et al. (2007). Structural insights into fibronectin type III domain-mediated signaling. J Mol Biol, 367, 303-309. PubMed id: 17261313

Date:

19-Mar-06

Release date:

17-Oct-06

PROCHECK

	Headers

	References

Protein chain

P02751 (FINC_HUMAN) - Fibronectin from Homo sapiens

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:

Seq: Struc:					2477 a.a. 188 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 11 residue positions (black crosses)

	J Mol Biol 367:303-309 (2007)
PubMed id: 17261313

Structural insights into fibronectin type III domain-mediated signaling.
S.Bencharit, C.B.Cui, A.Siddiqui, E.L.Howard-Williams, J.Sondek, K.Zuobi-Hasona, I.Aukhil.

ABSTRACT

The alternatively spliced type III extradomain B (EIIIB) of fibronectin (FN) is expressed only during embryogenesis, wound healing and tumorigenesis. The biological function of this domain is unclear. We describe here the first crystal structure of the interface between alternatively spliced EIIIB and its adjacent FN type III domain 8 (FN B-8). The opened CC' loop of EIIIB, and the rotation and tilt of EIIIB allow good access to the FG loop of FN-8, which is normally hindered by the CC' loop of FN-7. In addition, the AGEGIP sequence of the CC'' loop of EIIIB replaces the NGQQGN sequence of the CC' loop of FN-7. Finally, the CC'' loop of EIIIB forms an acidic groove with FN-8. These structural findings warrant future studies directed at identifying potential binding partners for FN B-8 interface, linking EIIIB to skeletal and cartilaginous development, wound healing, and tumorigenesis, respectively.

Literature references that cite this PDB file's key reference

PubMed id

Reference

20161770

E.Ventura, F.Sassi, A.Parodi, E.Balza, L.Borsi, P.Castellani, B.Carnemolla, and L.Zardi (2010).
Alternative splicing of the angiogenesis associated extra-domain B of fibronectin regulates the accessibility of the B-C loop of the type III repeat 8.

PLoS One, 5, e9145.

19479996

E.Balza, F.Sassi, E.Ventura, A.Parodi, S.Fossati, W.Blalock, B.Carnemolla, P.Castellani, L.Zardi, and L.Borsi (2009).
A novel human fibronectin cryptic sequence unmasked by the insertion of the angiogenesis-associated extra type III domain B.

Int J Cancer, 125, 751-758.

19193644

E.Ficko-Blean, K.J.Gregg, J.J.Adams, J.H.Hehemann, M.Czjzek, S.P.Smith, and A.B.Boraston (2009).
Portrait of an enzyme, a complete structural analysis of a multimodular {beta}-N-acetylglucosaminidase from Clostridium perfringens.

J Biol Chem, 284, 9876-9884.

PDB codes:

2v5c 2v5d 2w1n

19126199

K.Goossens, A.Van Soom, A.Van Zeveren, H.Favoreel, and L.J.Peelman (2009).
Quantification of fibronectin 1 (FN1) splice variants, including two novel ones, and analysis of integrins as candidate FN1 receptors in bovine preimplantation embryos.

BMC Dev Biol, 9, 1.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.