UniProt functional annotation for P46799

UniProt code: P46799.

Organism:		Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8).
Taxonomy:		Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga.
Function:		Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. {ECO:0000255|HAMAP-Rule:MF_00952}.
Catalytic activity:		Reaction=ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.; EC=5.6.2.1; Evidence={ECO:0000255|HAMAP- Rule:MF_00952};
Cofactor:		Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_00952}; Note=Binds two Mg(2+) per subunit. {ECO:0000255|HAMAP-Rule:MF_00952};
Subunit:		Monomer. {ECO:0000255|HAMAP-Rule:MF_00952}.
Similarity:		Belongs to the type IA topoisomerase family. {ECO:0000255|HAMAP-Rule:MF_00952}.

Annotations taken from UniProtKB at the EBI.