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PDBsum entry 2g9t
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Viral protein
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PDB id
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2g9t
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References listed in PDB file
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Key reference
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Title
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Dodecamer structure of severe acute respiratory syndrome coronavirus nonstructural protein nsp10.
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Authors
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D.Su,
Z.Lou,
F.Sun,
Y.Zhai,
H.Yang,
R.Zhang,
A.Joachimiak,
X.C.Zhang,
M.Bartlam,
Z.Rao.
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Ref.
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J Virol, 2006,
80,
7902-7908.
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PubMed id
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Abstract
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The severe acute respiratory syndrome coronavirus (SARS-CoV) nonstructural
proteins nsp1 to nsp16 have been implicated by genetic analysis in the assembly
of a functional replication/transcription complex. We report the crystal
structure of nsp10 from SARS-CoV at 2.1-A resolution. The nsp10 structure has a
novel fold, and 12 identical subunits assemble to form a unique spherical
dodecameric architecture. Two zinc fingers have been identified from the nsp10
monomer structure with the sequence motifs C-(X)2-C-(X)5-H-(X)6-C and
C-(X)2-C-(X)7-C-(X)-C. The nsp10 crystal structure is the first of a new class
of zinc finger protein three-dimensional structures to be revealed
experimentally. The zinc finger sequence motifs are conserved among all three
coronavirus antigenic groups, implicating an essential function for nsp10 in all
coronaviruses. Based on the structure, we propose that nsp10 is a transcription
factor for coronavirus replication/transcription.
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