UniProt functional annotation for P34216



	UniProt functional annotation for P34216

UniProt code: P34216.

Organism: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).

Taxonomy: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.

Function: Functions at the internalization step of the clathrin- mediated endocytosis (CME) as an early-acting scaffold protein. Requires clathrin adapter proteins, ENT1/2 and YAP1801/2, for normal spatiotemporal dynamics and viability. Binds to biological membranes in a ubiquitin-dependent manner. {ECO:0000269|PubMed:10954428, ECO:0000269|PubMed:12529323, ECO:0000269|PubMed:16239147, ECO:0000269|PubMed:18448668, ECO:0000269|PubMed:19713939, ECO:0000269|PubMed:19776351, ECO:0000269|PubMed:22190733}.

Subunit: Interacts (via UBA domain) with monoubiquitin and ENT1 (via asparagine-proline-phenylalanine tripeptide motif called NPF). Interacts with PAL1 and SYP1. {ECO:0000269|PubMed:12529323, ECO:0000269|PubMed:16563434, ECO:0000269|PubMed:19713939, ECO:0000269|PubMed:22190733}.

Subcellular location: Cytoplasm {ECO:0000269|PubMed:10954428, ECO:0000269|PubMed:11452010, ECO:0000269|PubMed:14562095, ECO:0000269|PubMed:18448668, ECO:0000269|PubMed:19713939, ECO:0000269|PubMed:19776351, ECO:0000269|PubMed:22190733}. Note=Localized to actin cortical patches concentrated in the developing bud tip in cells with small buds and at the mother-daughter neck in cells undergoing cytokinesis. Localization can be maintained in the absence of polymerized actin filaments.

Disruption phenotype: Random budding pattern and morphological defects. Defects in fluid-phase endocytosis and defective internalization of the pheromone alpha-factor and uracil permease. Deletion has only a small impact on actin cytoskeleton organization. Deletion shows synthetic growth defects with thermosensitive mutants of PAN1, END3 and RSP5. {ECO:0000269|PubMed:10954428, ECO:0000269|PubMed:11452010}.

Miscellaneous: Present with 1380 molecules/cell in log phase SD medium. {ECO:0000269|PubMed:14562106}.

Similarity: Belongs to the VDP/USO1/EDE1 family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
Taxonomy:		Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces.



Function:		Functions at the internalization step of the clathrin- mediated endocytosis (CME) as an early-acting scaffold protein. Requires clathrin adapter proteins, ENT1/2 and YAP1801/2, for normal spatiotemporal dynamics and viability. Binds to biological membranes in a ubiquitin-dependent manner. {ECO:0000269\|PubMed:10954428, ECO:0000269\|PubMed:12529323, ECO:0000269\|PubMed:16239147, ECO:0000269\|PubMed:18448668, ECO:0000269\|PubMed:19713939, ECO:0000269\|PubMed:19776351, ECO:0000269\|PubMed:22190733}.


Subunit:		Interacts (via UBA domain) with monoubiquitin and ENT1 (via asparagine-proline-phenylalanine tripeptide motif called NPF). Interacts with PAL1 and SYP1. {ECO:0000269\|PubMed:12529323, ECO:0000269\|PubMed:16563434, ECO:0000269\|PubMed:19713939, ECO:0000269\|PubMed:22190733}.
Subcellular location:		Cytoplasm {ECO:0000269\|PubMed:10954428, ECO:0000269\|PubMed:11452010, ECO:0000269\|PubMed:14562095, ECO:0000269\|PubMed:18448668, ECO:0000269\|PubMed:19713939, ECO:0000269\|PubMed:19776351, ECO:0000269\|PubMed:22190733}. Note=Localized to actin cortical patches concentrated in the developing bud tip in cells with small buds and at the mother-daughter neck in cells undergoing cytokinesis. Localization can be maintained in the absence of polymerized actin filaments.
Disruption phenotype:		Random budding pattern and morphological defects. Defects in fluid-phase endocytosis and defective internalization of the pheromone alpha-factor and uracil permease. Deletion has only a small impact on actin cytoskeleton organization. Deletion shows synthetic growth defects with thermosensitive mutants of PAN1, END3 and RSP5. {ECO:0000269\|PubMed:10954428, ECO:0000269\|PubMed:11452010}.
Miscellaneous:		Present with 1380 molecules/cell in log phase SD medium. {ECO:0000269\|PubMed:14562106}.
Similarity:		Belongs to the VDP/USO1/EDE1 family. {ECO:0000305}.