 |
PDBsum entry 2fbb
|
|
|
|
References listed in PDB file
|
 |
|
Key reference
|
|
|
Title
|
|
The structure of the hexagonal crystal form of hen egg-White lysozyme.
|
|
|
Authors
|
|
C.Brinkmann,
M.S.Weiss,
E.Weckert.
|
|
|
Ref.
|
|
Acta Crystallogr D Biol Crystallogr, 2006,
62,
349-355.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The three-dimensional structure of hen egg-white lysozyme (HEWL) in a hexagonal
crystal form has been determined and refined to 1.46 A resolution. This
hexagonal crystal form crystallizes from a saturated sodium nitrate solution at
pH 8.4. The crystals belong to space group P6(1)22, with unit-cell parameters a
= b = 85.64, c = 67.93 A. A total of 165 water molecules, 16 nitrate ions and
five sodium ions were located in the electron-density map. The hexagonal crystal
form exhibits a higher solvent content and a higher degree of disorder than
other crystal forms of lysozyme. The flexibility of the protein depends on the
crystal packing, although some residue ranges are flexible in all native HEWL
crystal forms.
|
|
|
|
|
|
|
|
Figure 2.
Figure 2 (a) and (b) Na132 and (c) and (d) Na133 in the
hexagonal HEWL crystal form. The twofold symmetry axes are
indicated. The distances between sodium and connected O atoms
are shown in (b) and (d), respectively. (2F[o] - F[c]) maps
contoured at 1.0  .
Distances are given in Å. Figures were generated using MOLSCRIPT
v2.1.2 (Kraulis, 1991[Kraulis, P. J. (1991). J. Appl. Cryst. 24,
946-950.]), BOBSCRIPT (Esnouf, 1997[Esnouf, R. M. (1997). J.
Mol. Graph. 15, 132-134.]) and RASTER3D v2.7b (Merritt & Bacon,
1997[Merritt, E. A. & Bacon, D. J. (1997). Methods Enzymol. 277,
505-524.]).
|
|
Figure 3.
Figure 3 C^  superposition
of HEWL crystal forms (triclinic, monoclinic, orthorhombic and
tetragonal structures; Brinkmann et al., 2006[Brinkmann, C.,
Weiss, M. S. & Weckert, E. (2006). In preparation.]) and of
HEWL, GEWL and TEWL. Calculations were performed using the
program LSQKAB (Collaborative Computional Project, Number 4,
1994[Collaborative Computational Project, Number 4 (1994). Acta
Cryst. D50, 760-763.]). Figures were generated using MOLSCRIPT
v2.1.2 (Kraulis, 1991[Kraulis, P. J. (1991). J. Appl. Cryst. 24,
946-950.]).
|
|
|
|
|
|
The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(2006,
62,
349-355)
copyright 2006.
|
|
|
|
|
|
|
