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PDBsum entry 2f26

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Hydrolase PDB id
2f26
Jmol
Contents
Protein chain
362 a.a.
Metals
_CL
Waters ×271
HEADER    HYDROLASE                               15-NOV-05   2F26
TITLE     CRYSTAL STRUCTURE OF THE HUMAN SIALIDASE NEU2 E111Q-Q112E
TITLE    2 DOUBLE MUTANT
COMPND    MOL_ID: 1;
COMPND   2 MOLECULE: SIALIDASE 2;
COMPND   3 CHAIN: A;
COMPND   4 SYNONYM: NEU2, CYTOSOLIC SIALIDASE, N-ACETYL-ALPHA-
COMPND   5 NEURAMINIDASE 2;
COMPND   6 EC: 3.2.1.18;
COMPND   7 ENGINEERED: YES;
COMPND   8 MUTATION: YES
SOURCE    MOL_ID: 1;
SOURCE   2 ORGANISM_SCIENTIFIC: HOMO SAPIENS;
SOURCE   3 ORGANISM_COMMON: HUMAN;
SOURCE   4 ORGANISM_TAXID: 9606;
SOURCE   5 EXPRESSION_SYSTEM: ESCHERICHIA COLI BL21;
SOURCE   6 EXPRESSION_SYSTEM_TAXID: 511693;
SOURCE   7 EXPRESSION_SYSTEM_STRAIN: BL21;
SOURCE   8 EXPRESSION_SYSTEM_VECTOR_TYPE: PLASMID;
SOURCE   9 EXPRESSION_SYSTEM_PLASMID: PGEX-2T
KEYWDS    SIALIDASE, NEURAMINIDASE, GANGLIOSIDE, DRUG DESIGN,
KEYWDS   2 HYDROLASE
EXPDTA    X-RAY DIFFRACTION
AUTHOR    L.M.G.CHAVAS,R.KATO,P.FUSI,C.TRINGALI,B.VENERANDO,
AUTHOR   2 G.TETTAMANTI,E.MONTI,S.WAKATSUKI
REVDAT   2   24-FEB-09 2F26    1       VERSN
REVDAT   1   21-NOV-06 2F26    0
JRNL        AUTH   L.M.G.CHAVAS,R.KATO,P.FUSI,C.TRINGALI,B.VENERANDO,
JRNL        AUTH 2 G.TETTAMANTI,E.MONTI,S.WAKATSUKI
JRNL        TITL   CRYSTAL STRUCTURE OF THE HUMAN SIALIDASE NEU2
JRNL        TITL 2 E111Q-Q112E DOUBLE MUTANT
JRNL        REF    TO BE PUBLISHED
JRNL        REFN
REMARK   1
REMARK   2
REMARK   2 RESOLUTION.    1.58 ANGSTROMS.
REMARK   3
REMARK   3 REFINEMENT.
REMARK   3   PROGRAM     : CNS 1.1
REMARK   3   AUTHORS     : BRUNGER,ADAMS,CLORE,DELANO,GROS,GROSSE-
REMARK   3               : KUNSTLEVE,JIANG,KUSZEWSKI,NILGES, PANNU,
REMARK   3               : READ,RICE,SIMONSON,WARREN
REMARK   3
REMARK   3  REFINEMENT TARGET : ENGH & HUBER
REMARK   3
REMARK   3  DATA USED IN REFINEMENT.
REMARK   3   RESOLUTION RANGE HIGH (ANGSTROMS) : 1.58
REMARK   3   RESOLUTION RANGE LOW  (ANGSTROMS) : 72.55
REMARK   3   DATA CUTOFF            (SIGMA(F)) : 0.000
REMARK   3   DATA CUTOFF HIGH         (ABS(F)) : NULL
REMARK   3   DATA CUTOFF LOW          (ABS(F)) : NULL
REMARK   3   COMPLETENESS (WORKING+TEST)   (%) : 99.5
REMARK   3   NUMBER OF REFLECTIONS             : 69226
REMARK   3
REMARK   3  FIT TO DATA USED IN REFINEMENT.
REMARK   3   CROSS-VALIDATION METHOD          : THROUGHOUT
REMARK   3   FREE R VALUE TEST SET SELECTION  : RANDOM
REMARK   3   R VALUE            (WORKING SET) : 0.209
REMARK   3   FREE R VALUE                     : 0.220
REMARK   3   FREE R VALUE TEST SET SIZE   (%) : 5.000
REMARK   3   FREE R VALUE TEST SET COUNT      : 3502
REMARK   3   ESTIMATED ERROR OF FREE R VALUE  : NULL
REMARK   3
REMARK   3  FIT IN THE HIGHEST RESOLUTION BIN.
REMARK   3   TOTAL NUMBER OF BINS USED           : NULL
REMARK   3   BIN RESOLUTION RANGE HIGH       (A) : NULL
REMARK   3   BIN RESOLUTION RANGE LOW        (A) : NULL
REMARK   3   BIN COMPLETENESS (WORKING+TEST) (%) : NULL
REMARK   3   REFLECTIONS IN BIN    (WORKING SET) : NULL
REMARK   3   BIN R VALUE           (WORKING SET) : NULL
REMARK   3   BIN FREE R VALUE                    : NULL
REMARK   3   BIN FREE R VALUE TEST SET SIZE  (%) : NULL
REMARK   3   BIN FREE R VALUE TEST SET COUNT     : NULL
REMARK   3   ESTIMATED ERROR OF BIN FREE R VALUE : NULL
REMARK   3
REMARK   3  NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT.
REMARK   3   PROTEIN ATOMS            : 2861
REMARK   3   NUCLEIC ACID ATOMS       : 0
REMARK   3   HETEROGEN ATOMS          : 1
REMARK   3   SOLVENT ATOMS            : 271
REMARK   3
REMARK   3  B VALUES.
REMARK   3   FROM WILSON PLOT           (A**2) : NULL
REMARK   3   MEAN B VALUE      (OVERALL, A**2) : 27.55
REMARK   3   OVERALL ANISOTROPIC B VALUE.
REMARK   3    B11 (A**2) : 4.75000
REMARK   3    B22 (A**2) : 4.75000
REMARK   3    B33 (A**2) : -9.49900
REMARK   3    B12 (A**2) : 1.50000
REMARK   3    B13 (A**2) : 0.00000
REMARK   3    B23 (A**2) : 0.00000
REMARK   3
REMARK   3  ESTIMATED COORDINATE ERROR.
REMARK   3   ESD FROM LUZZATI PLOT        (A) : NULL
REMARK   3   ESD FROM SIGMAA              (A) : NULL
REMARK   3   LOW RESOLUTION CUTOFF        (A) : NULL
REMARK   3
REMARK   3  CROSS-VALIDATED ESTIMATED COORDINATE ERROR.
REMARK   3   ESD FROM C-V LUZZATI PLOT    (A) : NULL
REMARK   3   ESD FROM C-V SIGMAA          (A) : NULL
REMARK   3
REMARK   3  RMS DEVIATIONS FROM IDEAL VALUES.
REMARK   3   BOND LENGTHS                 (A) : 0.005
REMARK   3   BOND ANGLES            (DEGREES) : 1.38
REMARK   3   DIHEDRAL ANGLES        (DEGREES) : NULL
REMARK   3   IMPROPER ANGLES        (DEGREES) : NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL MODEL : NULL
REMARK   3
REMARK   3  ISOTROPIC THERMAL FACTOR RESTRAINTS.    RMS    SIGMA
REMARK   3   MAIN-CHAIN BOND              (A**2) : NULL  ; NULL
REMARK   3   MAIN-CHAIN ANGLE             (A**2) : NULL  ; NULL
REMARK   3   SIDE-CHAIN BOND              (A**2) : NULL  ; NULL
REMARK   3   SIDE-CHAIN ANGLE             (A**2) : NULL  ; NULL
REMARK   3
REMARK   3  BULK SOLVENT MODELING.
REMARK   3   METHOD USED : NULL
REMARK   3   KSOL        : NULL
REMARK   3   BSOL        : 51.30
REMARK   3
REMARK   3  NCS MODEL : NULL
REMARK   3
REMARK   3  NCS RESTRAINTS.                         RMS   SIGMA/WEIGHT
REMARK   3   GROUP  1  POSITIONAL            (A) : NULL  ; NULL
REMARK   3   GROUP  1  B-FACTOR           (A**2) : NULL  ; NULL
REMARK   3
REMARK   3  PARAMETER FILE  1  : PROTEIN_REP.PARAM
REMARK   3  PARAMETER FILE  2  : WATER_REP.PARAM
REMARK   3  PARAMETER FILE  3  : CARBOHYDRATE.PARAM
REMARK   3  PARAMETER FILE  4  : ION.PARAM
REMARK   3  PARAMETER FILE  5  : NULL
REMARK   3  TOPOLOGY FILE  1   : NULL
REMARK   3  TOPOLOGY FILE  2   : NULL
REMARK   3  TOPOLOGY FILE  3   : NULL
REMARK   3  TOPOLOGY FILE  4   : NULL
REMARK   3  TOPOLOGY FILE  5   : NULL
REMARK   3
REMARK   3  OTHER REFINEMENT REMARKS: FOR CG1 AND CG2 OF VAL 12 AND 325,
REMARK   3  IT CAN NOT BE DISTINGUISHED WHICH OF CG1 OR CG2 IS IN AN
REMARK   3  ALTERNATED CONFIGURATION. OCCUPANCY(A) OF CG1+OCCUPANCY(A) OF
REMARK   3  CG2=1, OCCUPANCY(A) OF CG1+OCCUPANCY(B) OF CG2=1, AND
REMARK   3  OCCUPANCY(A) OF CG2+OCCUPANCY(B) OF CG2=1
REMARK   4
REMARK   4 2F26 COMPLIES WITH FORMAT V. 3.15, 01-DEC-08
REMARK 100
REMARK 100 THIS ENTRY HAS BEEN PROCESSED BY PDBJ ON 25-NOV-05.
REMARK 100 THE RCSB ID CODE IS RCSB035359.
REMARK 200
REMARK 200 EXPERIMENTAL DETAILS
REMARK 200  EXPERIMENT TYPE                : X-RAY DIFFRACTION
REMARK 200  DATE OF DATA COLLECTION        : 05-NOV-03
REMARK 200  TEMPERATURE           (KELVIN) : 100
REMARK 200  PH                             : 5.6
REMARK 200  NUMBER OF CRYSTALS USED        : 1
REMARK 200
REMARK 200  SYNCHROTRON              (Y/N) : Y
REMARK 200  RADIATION SOURCE               : PHOTON FACTORY
REMARK 200  BEAMLINE                       : BL-6A
REMARK 200  X-RAY GENERATOR MODEL          : NULL
REMARK 200  MONOCHROMATIC OR LAUE    (M/L) : M
REMARK 200  WAVELENGTH OR RANGE        (A) : 0.9780
REMARK 200  MONOCHROMATOR                  : SI(111)
REMARK 200  OPTICS                         : NULL
REMARK 200
REMARK 200  DETECTOR TYPE                  : CCD
REMARK 200  DETECTOR MANUFACTURER          : ADSC QUAMTUM 4R
REMARK 200  INTENSITY-INTEGRATION SOFTWARE : HKL-2000
REMARK 200  DATA SCALING SOFTWARE          : SCALEPACK
REMARK 200
REMARK 200  NUMBER OF UNIQUE REFLECTIONS   : 69226
REMARK 200  RESOLUTION RANGE HIGH      (A) : 1.580
REMARK 200  RESOLUTION RANGE LOW       (A) : 72.550
REMARK 200  REJECTION CRITERIA  (SIGMA(I)) : NULL
REMARK 200
REMARK 200 OVERALL.
REMARK 200  COMPLETENESS FOR RANGE     (%) : 99.5
REMARK 200  DATA REDUNDANCY                : NULL
REMARK 200  R MERGE                    (I) : 0.05300
REMARK 200  R SYM                      (I) : NULL
REMARK 200   FOR THE DATA SET  : 18.9000
REMARK 200
REMARK 200 IN THE HIGHEST RESOLUTION SHELL.
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE HIGH (A) : 1.58
REMARK 200  HIGHEST RESOLUTION SHELL, RANGE LOW  (A) : 1.64
REMARK 200  COMPLETENESS FOR SHELL     (%) : 97.6
REMARK 200  DATA REDUNDANCY IN SHELL       : NULL
REMARK 200  R MERGE FOR SHELL          (I) : 0.35100
REMARK 200  R SYM FOR SHELL            (I) : NULL
REMARK 200   FOR SHELL         : 6.600
REMARK 200
REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH
REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: MOLECULAR REPLACEMENT
REMARK 200 SOFTWARE USED: MOLREP
REMARK 200 STARTING MODEL: PDB ENTRY 1SNT
REMARK 200
REMARK 200 REMARK: NULL
REMARK 280
REMARK 280 CRYSTAL
REMARK 280 SOLVENT CONTENT, VS   (%): 61.70
REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 3.20
REMARK 280
REMARK 280 CRYSTALLIZATION CONDITIONS: SODIUM POTASSIUM PHOSPHATE, SODIUM
REMARK 280  CHLORIDE, PH 5.6, VAPOR DIFFUSION, HANGING DROP, TEMPERATURE
REMARK 280  289K
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY
REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: H 3
REMARK 290
REMARK 290      SYMOP   SYMMETRY
REMARK 290     NNNMMM   OPERATOR
REMARK 290       1555   X,Y,Z
REMARK 290       2555   -Y,X-Y,Z
REMARK 290       3555   -X+Y,-X,Z
REMARK 290       4555   X+2/3,Y+1/3,Z+1/3
REMARK 290       5555   -Y+2/3,X-Y+1/3,Z+1/3
REMARK 290       6555   -X+Y+2/3,-X+1/3,Z+1/3
REMARK 290       7555   X+1/3,Y+2/3,Z+2/3
REMARK 290       8555   -Y+1/3,X-Y+2/3,Z+2/3
REMARK 290       9555   -X+Y+1/3,-X+2/3,Z+2/3
REMARK 290
REMARK 290     WHERE NNN -> OPERATOR NUMBER
REMARK 290           MMM -> TRANSLATION VECTOR
REMARK 290
REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS
REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM
REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY
REMARK 290 RELATED MOLECULES.
REMARK 290   SMTRY1   1  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   1  0.000000  1.000000  0.000000        0.00000
REMARK 290   SMTRY3   1  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   2 -0.500000 -0.866025  0.000000        0.00000
REMARK 290   SMTRY2   2  0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3   2  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   3 -0.500000  0.866025  0.000000        0.00000
REMARK 290   SMTRY2   3 -0.866025 -0.500000  0.000000        0.00000
REMARK 290   SMTRY3   3  0.000000  0.000000  1.000000        0.00000
REMARK 290   SMTRY1   4  1.000000  0.000000  0.000000       72.82500
REMARK 290   SMTRY2   4  0.000000  1.000000  0.000000       42.04553
REMARK 290   SMTRY3   4  0.000000  0.000000  1.000000       21.40333
REMARK 290   SMTRY1   5 -0.500000 -0.866025  0.000000       72.82500
REMARK 290   SMTRY2   5  0.866025 -0.500000  0.000000       42.04553
REMARK 290   SMTRY3   5  0.000000  0.000000  1.000000       21.40333
REMARK 290   SMTRY1   6 -0.500000  0.866025  0.000000       72.82500
REMARK 290   SMTRY2   6 -0.866025 -0.500000  0.000000       42.04553
REMARK 290   SMTRY3   6  0.000000  0.000000  1.000000       21.40333
REMARK 290   SMTRY1   7  1.000000  0.000000  0.000000        0.00000
REMARK 290   SMTRY2   7  0.000000  1.000000  0.000000       84.09107
REMARK 290   SMTRY3   7  0.000000  0.000000  1.000000       42.80667
REMARK 290   SMTRY1   8 -0.500000 -0.866025  0.000000        0.00000
REMARK 290   SMTRY2   8  0.866025 -0.500000  0.000000       84.09107
REMARK 290   SMTRY3   8  0.000000  0.000000  1.000000       42.80667
REMARK 290   SMTRY1   9 -0.500000  0.866025  0.000000        0.00000
REMARK 290   SMTRY2   9 -0.866025 -0.500000  0.000000       84.09107
REMARK 290   SMTRY3   9  0.000000  0.000000  1.000000       42.80667
REMARK 290
REMARK 290 REMARK: NULL
REMARK 300
REMARK 300 BIOMOLECULE: 1
REMARK 300 SEE REMARK 350 FOR THE AUTHOR PROVIDED AND/OR PROGRAM
REMARK 300 GENERATED ASSEMBLY INFORMATION FOR THE STRUCTURE IN
REMARK 300 THIS ENTRY. THE REMARK MAY ALSO PROVIDE INFORMATION ON
REMARK 300 BURIED SURFACE AREA.
REMARK 300 REMARK: THE MONOMER IN THE ASYMMETRIC UNIT FORMS THE BIOLOGICAL
REMARK 300 UNIT
REMARK 350
REMARK 350 COORDINATES FOR A COMPLETE MULTIMER REPRESENTING THE KNOWN
REMARK 350 BIOLOGICALLY SIGNIFICANT OLIGOMERIZATION STATE OF THE
REMARK 350 MOLECULE CAN BE GENERATED BY APPLYING BIOMT TRANSFORMATIONS
REMARK 350 GIVEN BELOW.  BOTH NON-CRYSTALLOGRAPHIC AND
REMARK 350 CRYSTALLOGRAPHIC OPERATIONS ARE GIVEN.
REMARK 350
REMARK 350 BIOMOLECULE: 1
REMARK 350 AUTHOR DETERMINED BIOLOGICAL UNIT: MONOMERIC
REMARK 350 APPLY THE FOLLOWING TO CHAINS: A
REMARK 350   BIOMT1   1  1.000000  0.000000  0.000000        0.00000
REMARK 350   BIOMT2   1  0.000000  1.000000  0.000000        0.00000
REMARK 350   BIOMT3   1  0.000000  0.000000  1.000000        0.00000
REMARK 375
REMARK 375 SPECIAL POSITION
REMARK 375 THE FOLLOWING ATOMS ARE FOUND TO BE WITHIN 0.15 ANGSTROMS
REMARK 375 OF A SYMMETRY RELATED ATOM AND ARE ASSUMED TO BE ON SPECIAL
REMARK 375 POSITIONS.
REMARK 375
REMARK 375 ATOM RES CSSEQI
REMARK 375      HOH A1074  LIES ON A SPECIAL POSITION.
REMARK 375      HOH A1205  LIES ON A SPECIAL POSITION.
REMARK 465
REMARK 465 MISSING RESIDUES
REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE
REMARK 465 EXPERIMENT. (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 465 IDENTIFIER; SSSEQ=SEQUENCE NUMBER; I=INSERTION CODE.)
REMARK 465
REMARK 465   M RES C SSSEQI
REMARK 465     GLY A    -1
REMARK 465     SER A     0
REMARK 465     MET A     1
REMARK 465     ALA A     2
REMARK 465     SER A     3
REMARK 465     ALA A    42
REMARK 465     SER A    43
REMARK 465     LYS A    44
REMARK 465     LYS A    45
REMARK 465     ASP A    46
REMARK 465     GLU A    47
REMARK 465     HIS A    48
REMARK 465     ALA A    49
REMARK 465     SER A   284
REMARK 465     GLY A   285
REMARK 465     PRO A   286
REMARK 465     GLY A   287
REMARK 465     LEU A   378
REMARK 465     PRO A   379
REMARK 465     GLN A   380
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND LENGTHS
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,2(A3,1X,A1,I4,A1,1X,A4,3X),1X,F6.3)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   RES CSSEQI ATM2   DEVIATION
REMARK 500    VAL A  12   CB    VAL A  12   CG1     0.270
REMARK 500    VAL A  12   CB    VAL A  12   CG2    -0.236
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: COVALENT BOND ANGLES
REMARK 500
REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES
REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE
REMARK 500 THAN 6*RMSD (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN
REMARK 500 IDENTIFIER; SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT: (10X,I3,1X,A3,1X,A1,I4,A1,3(1X,A4,2X),12X,F5.1)
REMARK 500
REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999
REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996
REMARK 500
REMARK 500  M RES CSSEQI ATM1   ATM2   ATM3
REMARK 500    VAL A  12   CG1 -  CB  -  CG2 ANGL. DEV. =  32.8 DEGREES
REMARK 500    VAL A  12   CA  -  CB  -  CG1 ANGL. DEV. = -17.0 DEGREES
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: TORSION ANGLES
REMARK 500
REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS:
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500 STANDARD TABLE:
REMARK 500 FORMAT:(10X,I3,1X,A3,1X,A1,I4,A1,4X,F7.2,3X,F7.2)
REMARK 500
REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/PSI-
REMARK 500 CHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395 - 1400
REMARK 500
REMARK 500  M RES CSSEQI        PSI       PHI
REMARK 500    SER A  15      116.46   -165.29
REMARK 500    ILE A  22       71.69     60.18
REMARK 500    VAL A 212     -158.99    -93.57
REMARK 500    LEU A 217     -120.74   -129.26
REMARK 500    GLU A 228      -26.25   -151.58
REMARK 500    LEU A 240     -165.87   -101.80
REMARK 500    ASP A 306       73.01     57.32
REMARK 500    ALA A 333     -119.54   -130.64
REMARK 500
REMARK 500 REMARK: NULL
REMARK 500
REMARK 500 GEOMETRY AND STEREOCHEMISTRY
REMARK 500 SUBTOPIC: PLANAR GROUPS
REMARK 500
REMARK 500 PLANAR GROUPS IN THE FOLLOWING RESIDUES HAVE A TOTAL
REMARK 500 RMS DISTANCE OF ALL ATOMS FROM THE BEST-FIT PLANE
REMARK 500 BY MORE THAN AN EXPECTED VALUE OF 6*RMSD, WITH AN
REMARK 500 RMSD 0.02 ANGSTROMS, OR AT LEAST ONE ATOM HAS
REMARK 500 AN RMSD GREATER THAN THIS VALUE
REMARK 500 (M=MODEL NUMBER; RES=RESIDUE NAME; C=CHAIN IDENTIFIER;
REMARK 500 SSEQ=SEQUENCE NUMBER; I=INSERTION CODE).
REMARK 500
REMARK 500  M RES CSSEQI        RMS     TYPE
REMARK 500    TYR A 310         0.07    SIDE_CHAIN
REMARK 500
REMARK 500 REMARK: NULL
REMARK 800
REMARK 800 SITE
REMARK 800 SITE_IDENTIFIER: AC1
REMARK 800 EVIDENCE_CODE: SOFTWARE
REMARK 800 SITE_DESCRIPTION: BINDING SITE FOR RESIDUE CL A 1001
REMARK 900
REMARK 900 RELATED ENTRIES
REMARK 900 RELATED ID: 1SNT   RELATED DB: PDB
REMARK 900 HUMAN NEU2 APO-FORM
REMARK 900 RELATED ID: 1SO7   RELATED DB: PDB
REMARK 900 HUMAN NEU2 SUGAR-INDUCED FORM
REMARK 900 RELATED ID: 1VCU   RELATED DB: PDB
REMARK 900 HUMAN NEU2-DANA COMPLEX
REMARK 900 RELATED ID: 2F0Z   RELATED DB: PDB
REMARK 900 RELATED ID: 2F10   RELATED DB: PDB
REMARK 900 RELATED ID: 2F11   RELATED DB: PDB
REMARK 900 RELATED ID: 2F12   RELATED DB: PDB
REMARK 900 RELATED ID: 2F13   RELATED DB: PDB
REMARK 900 RELATED ID: 2F24   RELATED DB: PDB
REMARK 900 RELATED ID: 2F25   RELATED DB: PDB
REMARK 900 RELATED ID: 2F27   RELATED DB: PDB
REMARK 900 RELATED ID: 2F28   RELATED DB: PDB
REMARK 900 RELATED ID: 2F29   RELATED DB: PDB
DBREF  2F26 A    1   380  UNP    Q9Y3R4   NEUR2_HUMAN      1    380
SEQADV 2F26 GLY A   -1  UNP  Q9Y3R4              CLONING ARTIFACT
SEQADV 2F26 SER A    0  UNP  Q9Y3R4              CLONING ARTIFACT
SEQADV 2F26 GLN A  111  UNP  Q9Y3R4    GLU   111 ENGINEERED
SEQADV 2F26 GLU A  112  UNP  Q9Y3R4    GLN   112 ENGINEERED
SEQRES   1 A  382  GLY SER MET ALA SER LEU PRO VAL LEU GLN LYS GLU SER
SEQRES   2 A  382  VAL PHE GLN SER GLY ALA HIS ALA TYR ARG ILE PRO ALA
SEQRES   3 A  382  LEU LEU TYR LEU PRO GLY GLN GLN SER LEU LEU ALA PHE
SEQRES   4 A  382  ALA GLU GLN ARG ALA SER LYS LYS ASP GLU HIS ALA GLU
SEQRES   5 A  382  LEU ILE VAL LEU ARG ARG GLY ASP TYR ASP ALA PRO THR
SEQRES   6 A  382  HIS GLN VAL GLN TRP GLN ALA GLN GLU VAL VAL ALA GLN
SEQRES   7 A  382  ALA ARG LEU ASP GLY HIS ARG SER MET ASN PRO CYS PRO
SEQRES   8 A  382  LEU TYR ASP ALA GLN THR GLY THR LEU PHE LEU PHE PHE
SEQRES   9 A  382  ILE ALA ILE PRO GLY GLN VAL THR GLN GLU GLN GLN LEU
SEQRES  10 A  382  GLN THR ARG ALA ASN VAL THR ARG LEU CYS GLN VAL THR
SEQRES  11 A  382  SER THR ASP HIS GLY ARG THR TRP SER SER PRO ARG ASP
SEQRES  12 A  382  LEU THR ASP ALA ALA ILE GLY PRO ALA TYR ARG GLU TRP
SEQRES  13 A  382  SER THR PHE ALA VAL GLY PRO GLY HIS CYS LEU GLN LEU
SEQRES  14 A  382  ASN ASP ARG ALA ARG SER LEU VAL VAL PRO ALA TYR ALA
SEQRES  15 A  382  TYR ARG LYS LEU HIS PRO ILE GLN ARG PRO ILE PRO SER
SEQRES  16 A  382  ALA PHE CYS PHE LEU SER HIS ASP HIS GLY ARG THR TRP
SEQRES  17 A  382  ALA ARG GLY HIS PHE VAL ALA GLN ASP THR LEU GLU CYS
SEQRES  18 A  382  GLN VAL ALA GLU VAL GLU THR GLY GLU GLN ARG VAL VAL
SEQRES  19 A  382  THR LEU ASN ALA ARG SER HIS LEU ARG ALA ARG VAL GLN
SEQRES  20 A  382  ALA GLN SER THR ASN ASP GLY LEU ASP PHE GLN GLU SER
SEQRES  21 A  382  GLN LEU VAL LYS LYS LEU VAL GLU PRO PRO PRO GLN GLY
SEQRES  22 A  382  CYS GLN GLY SER VAL ILE SER PHE PRO SER PRO ARG SER
SEQRES  23 A  382  GLY PRO GLY SER PRO ALA GLN TRP LEU LEU TYR THR HIS
SEQRES  24 A  382  PRO THR HIS SER TRP GLN ARG ALA ASP LEU GLY ALA TYR
SEQRES  25 A  382  LEU ASN PRO ARG PRO PRO ALA PRO GLU ALA TRP SER GLU
SEQRES  26 A  382  PRO VAL LEU LEU ALA LYS GLY SER CYS ALA TYR SER ASP
SEQRES  27 A  382  LEU GLN SER MET GLY THR GLY PRO ASP GLY SER PRO LEU
SEQRES  28 A  382  PHE GLY CYS LEU TYR GLU ALA ASN ASP TYR GLU GLU ILE
SEQRES  29 A  382  VAL PHE LEU MET PHE THR LEU LYS GLN ALA PHE PRO ALA
SEQRES  30 A  382  GLU TYR LEU PRO GLN
HET     CL  A1001       1
HETNAM      CL CHLORIDE ION
FORMUL   2   CL    CL 1-
FORMUL   3  HOH   *271(H2 O)
HELIX    1   1 THR A  110  GLN A  116  1                                   7
HELIX    2   2 LEU A  142  GLY A  148  1                                   7
HELIX    3   3 PRO A  149  ARG A  152  5                                   4
HELIX    4   4 ALA A  317  TRP A  321  5                                   5
HELIX    5   5 LEU A  369  PHE A  373  1                                   5
HELIX    6   6 PRO A  374  TYR A  377  5                                   4
SHEET    1   A 4 GLN A   8  GLN A  14  0
SHEET    2   A 4 GLU A 361  THR A 368 -1  O  PHE A 364   N  GLU A  10
SHEET    3   A 4 PRO A 348  ALA A 356 -1  N  TYR A 354   O  VAL A 363
SHEET    4   A 4 SER A 335  THR A 342 -1  N  MET A 340   O  LEU A 349
SHEET    1   B 4 TYR A  20  LEU A  28  0
SHEET    2   B 4 SER A  33  GLN A  40 -1  O  GLU A  39   N  ARG A  21
SHEET    3   B 4 LEU A  51  ASP A  60 -1  O  LEU A  51   N  GLN A  40
SHEET    4   B 4 GLN A  65  TRP A  68 -1  O  GLN A  65   N  ASP A  60
SHEET    1   C 4 TYR A  20  LEU A  28  0
SHEET    2   C 4 SER A  33  GLN A  40 -1  O  GLU A  39   N  ARG A  21
SHEET    3   C 4 LEU A  51  ASP A  60 -1  O  LEU A  51   N  GLN A  40
SHEET    4   C 4 GLU A  72  VAL A  73 -1  O  GLU A  72   N  LEU A  54
SHEET    1   D 4 HIS A  82  TYR A  91  0
SHEET    2   D 4 LEU A  98  PRO A 106 -1  O  ILE A 105   N  ARG A  83
SHEET    3   D 4 ARG A 123  SER A 129 -1  O  CYS A 125   N  PHE A 102
SHEET    4   D 4 ARG A 140  ASP A 141 -1  O  ARG A 140   N  GLN A 126
SHEET    1   E 3 TRP A 154  VAL A 159  0
SHEET    2   E 3 LEU A 174  ARG A 182 -1  O  TYR A 181   N  THR A 156
SHEET    3   E 3 LEU A 165  GLN A 166 -1  N  LEU A 165   O  VAL A 175
SHEET    1   F 4 TRP A 154  VAL A 159  0
SHEET    2   F 4 LEU A 174  ARG A 182 -1  O  TYR A 181   N  THR A 156
SHEET    3   F 4 ILE A 191  SER A 199 -1  O  SER A 199   N  LEU A 174
SHEET    4   F 4 ALA A 207  ARG A 208 -1  O  ALA A 207   N  LEU A 198
SHEET    1   G 4 THR A 216  THR A 226  0
SHEET    2   G 4 GLN A 229  SER A 238 -1  O  THR A 233   N  ALA A 222
SHEET    3   G 4 ALA A 242  SER A 248 -1  O  VAL A 244   N  ALA A 236
SHEET    4   G 4 GLN A 259  VAL A 265 -1  O  GLN A 259   N  GLN A 245
SHEET    1   H 4 SER A 275  PRO A 280  0
SHEET    2   H 4 GLN A 291  PRO A 298 -1  O  TRP A 292   N  PHE A 279
SHEET    3   H 4 ALA A 305  ASN A 312 -1  O  TYR A 310   N  TYR A 295
SHEET    4   H 4 VAL A 325  SER A 331 -1  O  GLY A 330   N  ASP A 306
SSBOND   1 CYS A   88    CYS A  164                          1555   1555  2.77
CISPEP   1 GLY A  160    PRO A  161          0         0.49
CISPEP   2 PRO A  268    PRO A  269          0         0.09
CISPEP   3 PRO A  315    PRO A  316          0        -0.23
SITE     1 AC1  4 LEU A  28  GLY A  30  GLN A  31  HOH A1052
CRYST1  145.650  145.650   64.210  90.00  90.00 120.00 H 3           9
ORIGX1      1.000000  0.000000  0.000000        0.00000
ORIGX2      0.000000  1.000000  0.000000        0.00000
ORIGX3      0.000000  0.000000  1.000000        0.00000
SCALE1      0.006866  0.003964  0.000000        0.00000
SCALE2      0.000000  0.007928  0.000000        0.00000
SCALE3      0.000000  0.000000  0.015574        0.00000
      
PROCHECK
Go to PROCHECK summary
 References