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PDBsum entry 2ezd
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DNA binding protein/DNA
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PDB id
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2ezd
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References listed in PDB file
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Key reference
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Title
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The solution structure of an hmg-I(y)-Dna complex defines a new architectural minor groove binding motif.
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Authors
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J.R.Huth,
C.A.Bewley,
M.S.Nissen,
J.N.Evans,
R.Reeves,
A.M.Gronenborn,
G.M.Clore.
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Ref.
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Nat Struct Biol, 1997,
4,
657-665.
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PubMed id
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Abstract
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The solution structure of a complex between a truncated form of HMG-I(Y),
consisting of the second and third DNA binding domains (residues 51-90), and a
DNA dodecamer containing the PRDII site of the interferon-beta promoter has been
solved by multidimensional nuclear magnetic resonance spectroscopy. The
stoichiometry of the complex is one molecule of HMG-I(Y) to two molecules of
DNA. The structure reveals a new architectural minor groove binding motif which
stabilizes B-DNA, thereby facilitating the binding of other transcription
factors in the opposing major groove. The interactions involve a central
Arg-Gly-Arg motif together with two other modules that participate in extensive
hydrophobic and polar contracts. The absence of one of these modules in the
third DNA binding domain accounts for its-100 fold reduced affinity relative to
the second one.
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