UniProt functional annotation for P03681



	UniProt functional annotation for P03681

UniProt code: P03681.

Organism: Bacillus phage phi29 (Bacteriophage phi-29).

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Picovirinae; Salasvirus.

Function: Acts as a primer for DNA elongation during viral genomic replication (PubMed:6813861). Acts as the small terminase protein during packaging (PubMed:18674782). Recruits the phage DNA polymerase to the bacterial nucleoid (PubMed:20823229). Primer terminal protein (TP) is covalently linked to the 5'-ends of both strands of the genome through a phosphodiester bond between the beta-hydroxyl group of a serine residue and the 5'-phosphate of the terminal deoxyadenylate (dAMP) (PubMed:6813861). To start replication, the DNA polymerase forms a heterodimer with a free TP that recognizes the replication origins at both 5' ends of the linear chromosome, and initiates replication using as primer the OH-group of Ser-232 of the TP (PubMed:22210885, PubMed:25081208). Since the polymerase initiates the replication on the second thymine, the TP-dAMP initiation product slides backwards to recover the template information of the first nucleotide (PubMed:19011105). {ECO:0000269|PubMed:18674782, ECO:0000269|PubMed:19011105, ECO:0000269|PubMed:20823229, ECO:0000269|PubMed:22210885, ECO:0000269|PubMed:25081208, ECO:0000269|PubMed:6813861}.

Function: Hydrolyzes host peptidoglycans during virus entry. {ECO:0000269|PubMed:14763988}.

Subunit: Interacts with the viral polymerase; this interaction allows the initiation of TP-primed DNA replication at both viral DNA ends (PubMed:22210885). Binds to ssDNA (PubMed:6779279). Interacts with the replication protein p1 (PubMed:11032825). Part of a DNA-gp3-gp16 complex (PubMed:9086269). {ECO:0000269|PubMed:11032825, ECO:0000269|PubMed:22210885, ECO:0000269|PubMed:6779279, ECO:0000269|PubMed:9086269}.

Subcellular location: Virion {ECO:0000269|PubMed:14763988}. Host nucleus {ECO:0000269|PubMed:23091024}. Note=Associates with the host bacterial nucleoid through its N-terminal region. {ECO:0000269|PubMed:20823229}.

Similarity: Belongs to the phi29likevirus DNA terminal protein family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Bacillus phage phi29 (Bacteriophage phi-29).
Taxonomy:		Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Picovirinae; Salasvirus.



Function:		Acts as a primer for DNA elongation during viral genomic replication (PubMed:6813861). Acts as the small terminase protein during packaging (PubMed:18674782). Recruits the phage DNA polymerase to the bacterial nucleoid (PubMed:20823229). Primer terminal protein (TP) is covalently linked to the 5'-ends of both strands of the genome through a phosphodiester bond between the beta-hydroxyl group of a serine residue and the 5'-phosphate of the terminal deoxyadenylate (dAMP) (PubMed:6813861). To start replication, the DNA polymerase forms a heterodimer with a free TP that recognizes the replication origins at both 5' ends of the linear chromosome, and initiates replication using as primer the OH-group of Ser-232 of the TP (PubMed:22210885, PubMed:25081208). Since the polymerase initiates the replication on the second thymine, the TP-dAMP initiation product slides backwards to recover the template information of the first nucleotide (PubMed:19011105). {ECO:0000269\|PubMed:18674782, ECO:0000269\|PubMed:19011105, ECO:0000269\|PubMed:20823229, ECO:0000269\|PubMed:22210885, ECO:0000269\|PubMed:25081208, ECO:0000269\|PubMed:6813861}.



Function:		Hydrolyzes host peptidoglycans during virus entry. {ECO:0000269\|PubMed:14763988}.


Subunit:		Interacts with the viral polymerase; this interaction allows the initiation of TP-primed DNA replication at both viral DNA ends (PubMed:22210885). Binds to ssDNA (PubMed:6779279). Interacts with the replication protein p1 (PubMed:11032825). Part of a DNA-gp3-gp16 complex (PubMed:9086269). {ECO:0000269\|PubMed:11032825, ECO:0000269\|PubMed:22210885, ECO:0000269\|PubMed:6779279, ECO:0000269\|PubMed:9086269}.
Subcellular location:		Virion {ECO:0000269\|PubMed:14763988}. Host nucleus {ECO:0000269\|PubMed:23091024}. Note=Associates with the host bacterial nucleoid through its N-terminal region. {ECO:0000269\|PubMed:20823229}.
Similarity:		Belongs to the phi29likevirus DNA terminal protein family. {ECO:0000305}.