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PDBsum entry 2ewa
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Contents |
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* Residue conservation analysis
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PDB id:
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Transferase
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Title:
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Dual binding mode of pyridinylimidazole to map kinase p38
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Structure:
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Mitogen-activated protein kinase 14. Chain: a. Synonym: mitogen-activated protein kinase p38 alpha, map kinase p38 alpha, crk1. Engineered: yes
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Source:
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Mus musculus. House mouse. Organism_taxid: 10090. Gene: mapk14, crk1, csbp1, csbp2. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
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Resolution:
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2.10Å
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R-factor:
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0.241
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R-free:
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0.279
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Authors:
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L.Delarbre,S.Pouzieux,J.-P.Guilloteau,N.Michot
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Key ref:
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M.Vogtherr
et al.
(2006).
NMR characterization of kinase p38 dynamics in free and ligand-bound forms.
Angew Chem Int Ed Engl,
45,
993-997.
PubMed id:
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Date:
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02-Nov-05
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Release date:
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01-Aug-06
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PROCHECK
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Headers
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References
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P47811
(MK14_MOUSE) -
Mitogen-activated protein kinase 14 from Mus musculus
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Seq:
Struc:
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360 a.a.
330 a.a.*
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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*
PDB and UniProt seqs differ
at 2 residue positions (black
crosses)
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Enzyme class:
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E.C.2.7.11.24
- mitogen-activated protein kinase.
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Reaction:
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1.
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L-seryl-[protein] + ATP = O-phospho-L-seryl-[protein] + ADP + H+
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2.
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L-threonyl-[protein] + ATP = O-phospho-L-threonyl-[protein] + ADP + H+
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L-seryl-[protein]
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+
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ATP
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=
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O-phospho-L-seryl-[protein]
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+
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ADP
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+
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H(+)
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L-threonyl-[protein]
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+
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ATP
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=
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O-phospho-L-threonyl-[protein]
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+
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ADP
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+
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H(+)
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Molecule diagrams generated from .mol files obtained from the
KEGG ftp site
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Angew Chem Int Ed Engl
45:993-997
(2006)
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PubMed id:
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NMR characterization of kinase p38 dynamics in free and ligand-bound forms.
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M.Vogtherr,
K.Saxena,
S.Hoelder,
S.Grimme,
M.Betz,
U.Schieborr,
B.Pescatore,
M.Robin,
L.Delarbre,
T.Langer,
K.U.Wendt,
H.Schwalbe.
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ABSTRACT
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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B.Yu,
I.R.Martins,
P.Li,
G.K.Amarasinghe,
J.Umetani,
M.E.Fernandez-Zapico,
D.D.Billadeau,
M.Machius,
D.R.Tomchick,
and
M.K.Rosen
(2010).
Structural and energetic mechanisms of cooperative autoinhibition and activation of Vav1.
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Cell,
140,
246-256.
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PDB code:
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J.M.Steichen,
G.H.Iyer,
S.Li,
S.A.Saldanha,
M.S.Deal,
V.L.Woods,
and
S.S.Taylor
(2010).
Global consequences of activation loop phosphorylation on protein kinase A.
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J Biol Chem,
285,
3825-3832.
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L.M.Wodicka,
P.Ciceri,
M.I.Davis,
J.P.Hunt,
M.Floyd,
S.Salerno,
X.H.Hua,
J.M.Ford,
R.C.Armstrong,
P.P.Zarrinkar,
and
D.K.Treiber
(2010).
Activation state-dependent binding of small molecule kinase inhibitors: structural insights from biochemistry.
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Chem Biol,
17,
1241-1249.
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M.Rabiller,
M.Getlik,
S.Klüter,
A.Richters,
S.Tückmantel,
J.R.Simard,
and
D.Rauh
(2010).
Proteus in the world of proteins: conformational changes in protein kinases.
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Arch Pharm (Weinheim),
343,
193-206.
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R.Akella,
X.Min,
Q.Wu,
K.H.Gardner,
and
E.J.Goldsmith
(2010).
The third conformation of p38α MAP kinase observed in phosphorylated p38α and in solution.
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Structure,
18,
1571-1578.
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PDB code:
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R.S.Armen,
J.Chen,
and
C.L.Brooks
(2009).
An Evaluation of Explicit Receptor Flexibility in Molecular Docking Using Molecular Dynamics and Torsion Angle Molecular Dynamics.
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J Chem Theory Comput,
5,
2909-2923.
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Y.Li,
and
A.G.Palmer
(2009).
Domain swapping in the kinase superfamily: OSR1 joins the mix.
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Protein Sci,
18,
678-681.
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Y.Shan,
M.A.Seeliger,
M.P.Eastwood,
F.Frank,
H.Xu,
M.Ã.˜.Jensen,
R.O.Dror,
J.Kuriyan,
and
D.E.Shaw
(2009).
A conserved protonation-dependent switch controls drug binding in the Abl kinase.
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Proc Natl Acad Sci U S A,
106,
139-144.
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K.Mihara,
C.Almansa,
R.L.Smeets,
E.E.Loomans,
J.Dulos,
P.M.Vink,
M.Rooseboom,
H.Kreutzer,
F.Cavalcanti,
A.M.Boots,
and
R.L.Nelissen
(2008).
A potent and selective p38 inhibitor protects against bone damage in murine collagen-induced arthritis: a comparison with neutralization of mouse TNFalpha.
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Br J Pharmacol,
154,
153-164.
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J.Eswaran,
W.H.Lee,
J.E.Debreczeni,
P.Filippakopoulos,
A.Turnbull,
O.Fedorov,
S.W.Deacon,
J.R.Peterson,
and
S.Knapp
(2007).
Crystal Structures of the p21-activated kinases PAK4, PAK5, and PAK6 reveal catalytic domain plasticity of active group II PAKs.
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Structure,
15,
201-213.
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PDB codes:
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M.Tonelli,
L.R.Masterson,
K.Hallenga,
G.Veglia,
and
J.L.Markley
(2007).
Carbonyl carbon label selective (CCLS) 1H-15N HSQC experiment for improved detection of backbone 13C-15N cross peaks in larger proteins.
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J Biomol NMR,
39,
177-185.
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N.M.Levinson,
O.Kuchment,
K.Shen,
M.A.Young,
M.Koldobskiy,
M.Karplus,
P.A.Cole,
and
J.Kuriyan
(2006).
A Src-like inactive conformation in the abl tyrosine kinase domain.
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PLoS Biol,
4,
e144.
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PDB codes:
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
