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UniProt functional annotation for Q8NEJ0 | ||
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| UniProt code: Q8NEJ0. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Can dephosphorylate single and diphosphorylated synthetic MAPK peptides, with preference for the phosphotyrosine and diphosphorylated forms over phosphothreonine. In vitro, dephosphorylates p-nitrophenyl phosphate (pNPP). {ECO:0000269|PubMed:12408986, ECO:0000269|PubMed:12591617}. |
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| Catalytic activity: | |
Reaction=H2O + O-phospho-L-tyrosyl-[protein] = L-tyrosyl-[protein] + phosphate; Xref=Rhea:RHEA:10684, Rhea:RHEA-COMP:10136, Rhea:RHEA- COMP:10137, ChEBI:CHEBI:15377, ChEBI:CHEBI:43474, ChEBI:CHEBI:46858, ChEBI:CHEBI:82620; EC=3.1.3.48; Evidence={ECO:0000255|PROSITE- ProRule:PRU10044, ECO:0000269|PubMed:12408986, ECO:0000269|PubMed:12591617}; |
| Catalytic activity: | |
Reaction=H2O + O-phospho-L-seryl-[protein] = L-seryl-[protein] + phosphate; Xref=Rhea:RHEA:20629, Rhea:RHEA-COMP:9863, Rhea:RHEA- COMP:11604, ChEBI:CHEBI:15377, ChEBI:CHEBI:29999, ChEBI:CHEBI:43474, ChEBI:CHEBI:83421; EC=3.1.3.16; Evidence={ECO:0000269|PubMed:12408986, ECO:0000269|PubMed:12591617}; |
| Catalytic activity: | |
Reaction=H2O + O-phospho-L-threonyl-[protein] = L-threonyl-[protein] + phosphate; Xref=Rhea:RHEA:47004, Rhea:RHEA-COMP:11060, Rhea:RHEA- COMP:11605, ChEBI:CHEBI:15377, ChEBI:CHEBI:30013, ChEBI:CHEBI:43474, ChEBI:CHEBI:61977; EC=3.1.3.16; Evidence={ECO:0000269|PubMed:12408986, ECO:0000269|PubMed:12591617}; |
| Activity regulation: | |
Activated by manganese ions, inhibited by iodoaretic acid. {ECO:0000269|PubMed:12591617}. |
| Biophysicochemical properties: | |
pH dependence: Optimum pH is 6.0. {ECO:0000269|PubMed:12591617}; Temperature dependence: Optimum temperature is 55 degrees Celsius. {ECO:0000269|PubMed:12591617}; |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:12408986}. Nucleus {ECO:0000269|PubMed:12408986}. Mitochondrion inner membrane {ECO:0000250|UniProtKB:Q8VE01}; Peripheral membrane protein {ECO:0000250|UniProtKB:Q8VE01}; Intermembrane side {ECO:0000250|UniProtKB:Q8VE01}. Note=Translocates to cytoplasm in response to apoptotic stimuli such as staurosporine treatment. {ECO:0000250|UniProtKB:Q8VE01}. |
| Tissue specificity: | |
Widely expressed with highest levels in liver, brain, ovary and testis. {ECO:0000269|PubMed:12408986, ECO:0000269|PubMed:12591617}. |
| Similarity: | |
Belongs to the protein-tyrosine phosphatase family. Non- receptor class dual specificity subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.