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UniProt functional annotation for Q9DGB9 | ||
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| UniProt code: Q9DGB9. |
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| Organism: | |
Crotalus atrox (Western diamondback rattlesnake). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera; Serpentes; Colubroidea; Viperidae; Crotalinae; Crotalus. |
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| Function: | |
Zinc metalloprotease that has fibrinogenolytic and hemorrhagic activities. It induces apoptosis in vascular endothelial cells (VEC), without degrading extracellular matrix (it cannot cleave collagen) or inhibiting adhesion of VEC. VAP1-induced apoptosis is inhibited by antibodies for integrin alpha-3, alpha-6, beta-1 and CD9. Apoptosis is accompanied by severe cell fragmentation, which is controlled by caspases. {ECO:0000269|PubMed:11071872, ECO:0000269|PubMed:11821125, ECO:0000269|PubMed:15922392, ECO:0000269|PubMed:18657564, ECO:0000269|PubMed:9196035}. |
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| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000250}; Note=Binds 1 zinc ion per subunit. {ECO:0000250}; |
| Activity regulation: | |
Inhibited by EDTA and EGTA, but not by PMSF. {ECO:0000269|PubMed:11071872}. |
| Subunit: | |
Homodimer; disulfide-linked. {ECO:0000269|PubMed:16688218, ECO:0000269|PubMed:9196035}. |
| Subcellular location: | |
Secreted {ECO:0000250}. |
| Tissue specificity: | |
Expressed by the venom gland. |
| Ptm: | |
The N-terminus is blocked. {ECO:0000269|PubMed:19371136}. |
| Toxic dose: | |
LD(50) is 0.3 ug/ml against vascular endothelial cells in culture. {ECO:0000269|PubMed:9196035}. |
| Similarity: | |
Belongs to the venom metalloproteinase (M12B) family. P-III subfamily. P-IIIc sub-subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.