PDBsum entry 2ekk

Protein binding

PDB id: 2ekk

Contents

Protein chain

47 a.a.

PDB id:

2ekk

Name:

Protein binding

Title:

Solution structure of ruh-074, a human uba domain

Structure:

Uba domain from e3 ubiquitin-protein ligase huwe1. Chain: a. Synonym: hect, uba and wwe domain-containing protein 1, ure-b1, mcl-1 ubiquitin ligase e3, mule, arf-binding protein 1, arf-bp1. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Other_details: cell-free protein synthesis

NMR struc:

20 models

Authors:

S.Kitasaka,A.Z.M.Ruhul Momen,H.Hirota,Y.Muto,S.Yokoyama,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

S.Kitasaka et al. Solution structure of ruh-074, A human uba domain. To be published, .

Date:

23-Mar-07 Release date: 25-Sep-07

Protein chain

Q7Z6Z7  (HUWE1_HUMAN) -  E3 ubiquitin-protein ligase HUWE1 from Homo sapiens

Seq: 4374 a.a.

Struc: 47 a.a.*

* PDB and UniProt seqs differ at 5 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine