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UniProt functional annotation for Q9LDI3 | ||
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| UniProt code: Q9LDI3. |
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| Organism: | |
Arabidopsis thaliana (Mouse-ear cress). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. |
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| Function: | |
Involved in the regulatory pathway for the control of intracellular Na(+) and K(+) homeostasis and salt tolerance. Activates the vacuolar H(+)/Ca(2+) antiporter CAX1 and operates in synergy with CBL4/SOS3 to activate the plasma membrane Na(+)/H(+) antiporter SOS1. CIPK serine-threonine protein kinases interact with CBL proteins. Binding of a CBL protein to the regulatory NAF domain of CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Phosphorylates CBL1, CBL4 and CBL10. {ECO:0000269|PubMed:10725350, ECO:0000269|PubMed:12034882, ECO:0000269|PubMed:14583601, ECO:0000269|PubMed:22253446}. |
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| Catalytic activity: | |
Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl- [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA- COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence={ECO:0000269|PubMed:22253446}; |
| Catalytic activity: | |
Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L- threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence={ECO:0000269|PubMed:22253446}; |
| Cofactor: | |
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:22253446}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=131.2 uM for synthetic substrate {ECO:0000269|PubMed:22253446}; Vmax=1304 pmol/min/mg enzyme {ECO:0000269|PubMed:22253446}; |
| Subunit: | |
Interacts with CBL1, CBL2, CBL4/SOS3, CBL5, CBL9, CBL10 and with the protein phosphatase 2C ABI2. {ECO:0000269|PubMed:10725350, ECO:0000269|PubMed:14504388, ECO:0000269|PubMed:14730064, ECO:0000269|PubMed:17499048, ECO:0000269|PubMed:17825054, ECO:0000269|PubMed:22253446}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:17825054}. Nucleus {ECO:0000269|PubMed:17825054}. Note=Targeted to the cell membrane when interacting with CBL1 or CBL5 and to the tonoplast when interacting with CBL2 orCBL10. |
| Induction: | |
Up-regulated in roots by salt stress. |
| Domain: | |
The activation loop within the kinase domain is the target of phosphorylation/activation by upstream protein kinases. The PPI motif mediates the interaction with the ABI (abscisic acid-insensitive) phosphatases. The C-terminal part (309-446) of the protein is required for the phosphorylation of CBL, but is not involved in autophosphorylation. {ECO:0000269|PubMed:22253446}. |
| Ptm: | |
Autophosphorylated. {ECO:0000269|PubMed:22253446}. |
| Similarity: | |
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily. {ECO:0000305}. |
| Sequence caution: | |
Sequence=AAF67384.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.