UniProt functional annotation for O81223



	UniProt functional annotation for O81223

UniProt code: O81223.

Organism: Arabidopsis thaliana (Mouse-ear cress).

Taxonomy: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.

Function: Acts as a calcium sensor involved in the regulatory pathway for the control of intracellular Na(+) and K(+) homeostasis and salt tolerance. Binding of a CBL protein to the regulatory NAF domain of a CIPK serine-threonine protein kinase lead to the activation of the kinase in a calcium-dependent manner. Operates in synergy with CIPK24/SOS2 to activate the plasma membrane Na(+)/H(+) antiporter SOS1. Involved in salt stress responses by mediating calcium-dependent microfilament reorganization. The CBL4/CIPK6 complex mediates translocation of AKT2 from the endoplasmic reticulum to the plasma membrane. Both myristoylation and S-acylation are required for AKT2 activation. {ECO:0000269|PubMed:10725350, ECO:0000269|PubMed:11006339, ECO:0000269|PubMed:12034882, ECO:0000269|PubMed:21445098, ECO:0000269|PubMed:23052592}.

Cofactor: Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 4 Ca(2+) ions per subunit.;

Subunit: Interacts with CIPK24/SOS2, CIPK6/SIP3, CIPK10/SIP1, CIPK11/SIP4 and CIPK15/SIP2. Homodimer, mediated by calcium-binding. Binds to ABCG36 (PubMed:26315018). {ECO:0000269|PubMed:10590166, ECO:0000269|PubMed:10725350, ECO:0000269|PubMed:15644219, ECO:0000269|PubMed:17499048, ECO:0000269|PubMed:21445098, ECO:0000269|PubMed:22253446, ECO:0000269|PubMed:26315018}.

Subcellular location: Cell membrane {ECO:0000269|PubMed:19832944}; Lipid-anchor {ECO:0000269|PubMed:19832944}. Cytoplasm {ECO:0000269|PubMed:19832944}. Nucleus {ECO:0000269|PubMed:19832944}. Note=The cell membrane localization is S-acylation dependent.

Domain: The calcium-binding domain (165-176) of the EF-hand 4 can also interacts with a manganese ion. The N-terminal 18 amino acids are sufficient for the cell membrane targeting of a heterologous protein. {ECO:0000269|PubMed:19832944}.

Ptm: Both N-myristoylation and calcium-mediated conformational changes are essential for its function. S-acylated at Cys-3. Phosphorylated by CIPK6 and CIPK24. {ECO:0000269|PubMed:11006339, ECO:0000269|PubMed:18502848, ECO:0000269|PubMed:21445098, ECO:0000269|PubMed:22253446}.

Disruption phenotype: Delayed development and flowering. {ECO:0000269|PubMed:21445098}.

Similarity: Belongs to the calcineurin regulatory subunit family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Arabidopsis thaliana (Mouse-ear cress).
Taxonomy:		Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.



Function:		Acts as a calcium sensor involved in the regulatory pathway for the control of intracellular Na(+) and K(+) homeostasis and salt tolerance. Binding of a CBL protein to the regulatory NAF domain of a CIPK serine-threonine protein kinase lead to the activation of the kinase in a calcium-dependent manner. Operates in synergy with CIPK24/SOS2 to activate the plasma membrane Na(+)/H(+) antiporter SOS1. Involved in salt stress responses by mediating calcium-dependent microfilament reorganization. The CBL4/CIPK6 complex mediates translocation of AKT2 from the endoplasmic reticulum to the plasma membrane. Both myristoylation and S-acylation are required for AKT2 activation. {ECO:0000269\|PubMed:10725350, ECO:0000269\|PubMed:11006339, ECO:0000269\|PubMed:12034882, ECO:0000269\|PubMed:21445098, ECO:0000269\|PubMed:23052592}.


Cofactor:		Name=Ca(2+); Xref=ChEBI:CHEBI:29108; Note=Binds 4 Ca(2+) ions per subunit.;
Subunit:		Interacts with CIPK24/SOS2, CIPK6/SIP3, CIPK10/SIP1, CIPK11/SIP4 and CIPK15/SIP2. Homodimer, mediated by calcium-binding. Binds to ABCG36 (PubMed:26315018). {ECO:0000269\|PubMed:10590166, ECO:0000269\|PubMed:10725350, ECO:0000269\|PubMed:15644219, ECO:0000269\|PubMed:17499048, ECO:0000269\|PubMed:21445098, ECO:0000269\|PubMed:22253446, ECO:0000269\|PubMed:26315018}.
Subcellular location:		Cell membrane {ECO:0000269\|PubMed:19832944}; Lipid-anchor {ECO:0000269\|PubMed:19832944}. Cytoplasm {ECO:0000269\|PubMed:19832944}. Nucleus {ECO:0000269\|PubMed:19832944}. Note=The cell membrane localization is S-acylation dependent.
Domain:		The calcium-binding domain (165-176) of the EF-hand 4 can also interacts with a manganese ion. The N-terminal 18 amino acids are sufficient for the cell membrane targeting of a heterologous protein. {ECO:0000269\|PubMed:19832944}.
Ptm:		Both N-myristoylation and calcium-mediated conformational changes are essential for its function. S-acylated at Cys-3. Phosphorylated by CIPK6 and CIPK24. {ECO:0000269\|PubMed:11006339, ECO:0000269\|PubMed:18502848, ECO:0000269\|PubMed:21445098, ECO:0000269\|PubMed:22253446}.
Disruption phenotype:		Delayed development and flowering. {ECO:0000269\|PubMed:21445098}.
Similarity:		Belongs to the calcineurin regulatory subunit family. {ECO:0000305}.