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PDBsum entry 2ehb
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Signalling protein/transferase
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PDB id
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2ehb
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References listed in PDB file
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Key reference
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Title
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The structure of the c-Terminal domain of the protein kinase atsos2 bound to the calcium sensor atsos3.
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Authors
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M.J.Sánchez-Barrena,
H.Fujii,
I.Angulo,
M.Martínez-Ripoll,
J.K.Zhu,
A.Albert.
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Ref.
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Mol Cell, 2007,
26,
427-435.
[DOI no: ]
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PubMed id
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Abstract
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The plant SOS2 family of protein kinases and their interacting activators, the
SOS3 family of calcium-binding proteins, function together in decoding calcium
signals elicited by different environmental stimuli. SOS2 is activated by
Ca-SOS3 and subsequently phosphorylates the ion transporter SOS1 to bring about
cellular ion homeostasis under salt stress. In addition to possessing the kinase
activity, members of the SOS2 family of protein kinases can bind to protein
phosphatase 2Cs. The crystal structure of the binary complex of Ca-SOS3 with the
C-terminal regulatory moiety of SOS2 resolves central questions regarding the
dual function of SOS2 as a kinase and a phosphatase-binding protein. A
comparison with the structure of unbound SOS3 reveals the basis of the molecular
function of this family of kinases and their interacting calcium sensors.
Furthermore, our study suggests that the structure of the
phosphatase-interaction domain of SOS2 defines a scaffold module conserved from
yeast to human.
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Figure 2.
Figure 2. Structural Comparison of SOS3 and Related
Structures
(A) Stereoview of the superimposition of the
ribbon diagrams corresponding to the structures of the
SOS3-FISL/NAF domain (orange/blue) and the unbound form of SOS3
(green) (PDB code 1V1G).
(B) Structure of the
SOS3-FISL/FISL domain (left) and the structurally related
complexes is shown: the regulatory and catalytic subunits of
calcineurin (center, PDB code 1TCO) and the cacineurin B
homologous protein and the plasma membrane Na/H exchanger
(right, PDB code 2BEC).
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Figure 5.
Figure 5. Relevant Interactions between the SOS3-FISL/NAF
Domain and the PPI Domain
A section of the structure of the
regulatory domain of SOS2 and SOS3 showing the residues
interacting with Arg340. The labeled residues are within 3.8
Å sphere around Arg340. The straight dashed lines stand
for the hydrogen bonds. Residues belonging to the SOS3-FISL/NAF
domain are depicted in green. Residues belonging to the PPI
domain are depicted in orange.
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The above figures are
reprinted
by permission from Cell Press:
Mol Cell
(2007,
26,
427-435)
copyright 2007.
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