PDBsum entry 2efh

Transport protein

PDB id: 2efh

Contents

Protein chains

246 a.a. *

152 a.a. *

Ligands

GDP ×2

Waters ×321

* Residue conservation analysis

PDB id:

2efh

Name:

Transport protein

Title:

Ara7-gdp/atvps9a(d185n)

Structure:

Similarity to vacuolar protein sorting-associated protein vps9. Chain: a, c. Fragment: vps9 domain. Synonym: atvps9a, hypothetical protein at3g19770. Engineered: yes. Mutation: yes. Small gtp-binding protein-like. Chain: b, d.

Source:

Arabidopsis thaliana. Thale cress. Organism_taxid: 3702. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

2.10Å R-factor: 0.216 R-free: 0.259

Authors:

K.Ihara,T.Uejima,S.Wakatsuki

Key ref:

T.Uejima et al. (2010). GDP-bound and nucleotide-free intermediates of the guanine nucleotide exchange in the Rab5·Vps9 system. J Biol Chem, 285, 36689-36697. PubMed id: 20833725

Date:

22-Feb-07 Release date: 26-Feb-08

Protein chains

Q9LT31  (VPS9A_ARATH) -  Vacuolar protein sorting-associated protein 9A from Arabidopsis thaliana

Seq: 520 a.a.

Struc: 246 a.a.*

Protein chains

Q9SN68  (RAF2B_ARATH) -  Ras-related protein RABF2b from Arabidopsis thaliana

Seq: 200 a.a.

Struc: 152 a.a.

* PDB and UniProt seqs differ at 1 residue position (black cross)

Enzyme reactions

• Enzyme class: Chains A, B, C, D: E.C.?

J Biol Chem 285:36689-36697 (2010)

PubMed id: 20833725

GDP-bound and nucleotide-free intermediates of the guanine nucleotide exchange in the Rab5·Vps9 system.

T.Uejima, K.Ihara, T.Goh, E.Ito, M.Sunada, T.Ueda, A.Nakano, S.Wakatsuki.

ABSTRACT

No abstract given.