UniProt functional annotation for Q9SN68



	UniProt functional annotation for Q9SN68

UniProt code: Q9SN68.

Organism: Arabidopsis thaliana (Mouse-ear cress).

Taxonomy: Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.

Function: Endosomal protein that may be involved in endocytosis (PubMed:16103374). Involved in the trafficking of proteins from prevacuolar compartments (PVCs) to vacuoles (PubMed:23682115, PubMed:24824487). May activate the MON1-CCZ1 complex which acts as guanine nucleotide exchange factors (GEF) for Rab7 protein family, and serves as a link between Rab5 and Rab7 families in PVCs, and mediates PVC maturation (PubMed:24824487). Involved in vacuolar transport of storage proteins with EREX as effector. Regulates membrane trafficking to protein storage vacuoles (PSVs) (PubMed:27288222). {ECO:0000269|PubMed:16103374, ECO:0000269|PubMed:23682115, ECO:0000269|PubMed:24824487, ECO:0000269|PubMed:27288222}.

Activity regulation: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. {ECO:0000305|PubMed:24824487}.

Subunit: Interacts with VPS9A homodimer (PubMed:18055610, PubMed:20833725). Interacts with TCTP1 (PubMed:20736351). Interacts with MON1 (PubMed:24824487). Interacts with EREX (via PX domain) (PubMed:27288222). Binds to VPS3 (PubMed:29463724). {ECO:0000269|PubMed:18055610, ECO:0000269|PubMed:20736351, ECO:0000269|PubMed:20833725, ECO:0000269|PubMed:24824487, ECO:0000269|PubMed:27288222, ECO:0000269|PubMed:29463724}.

Subcellular location: Early endosome membrane {ECO:0000269|PubMed:11532937, ECO:0000269|PubMed:23482856}; Lipid- anchor {ECO:0000305}. Endosome membrane {ECO:0000269|PubMed:15608333, ECO:0000269|PubMed:16103374, ECO:0000269|PubMed:24824487}; Lipid-anchor {ECO:0000305}. Prevacuolar compartment membrane {ECO:0000269|PubMed:15509844, ECO:0000269|PubMed:24824487}; Lipid- anchor {ECO:0000305}. Endosome, multivesicular body membrane {ECO:0000269|PubMed:23682115}; Lipid-anchor {ECO:0000305}. Cell membrane {ECO:0000305|PubMed:23519409}; Lipid-anchor {ECO:0000305}. Cytoplasm {ECO:0000269|PubMed:29463724}. Note=Strong co-localization with VPS3 and VPS18 in cytoplasmic puncta. {ECO:0000269|PubMed:29463724}.

Tissue specificity: Expressed in roots and actively dividing cells. {ECO:0000269|PubMed:11532937}.

Developmental stage: Expressed during pollen germination and pollen tube growth. {ECO:0000269|PubMed:18775970}.

Induction: Activated by VPS9A. {ECO:0000269|PubMed:18055610}.

Miscellaneous: Over-expression of the constitutively active GTP-bound mutant of Leu-69 induces the formation of large ring-like structures of 1-2 micrometers in diameter. {ECO:0000269|PubMed:23682115}.

Similarity: Belongs to the small GTPase superfamily. Rab family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Arabidopsis thaliana (Mouse-ear cress).
Taxonomy:		Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.



Function:		Endosomal protein that may be involved in endocytosis (PubMed:16103374). Involved in the trafficking of proteins from prevacuolar compartments (PVCs) to vacuoles (PubMed:23682115, PubMed:24824487). May activate the MON1-CCZ1 complex which acts as guanine nucleotide exchange factors (GEF) for Rab7 protein family, and serves as a link between Rab5 and Rab7 families in PVCs, and mediates PVC maturation (PubMed:24824487). Involved in vacuolar transport of storage proteins with EREX as effector. Regulates membrane trafficking to protein storage vacuoles (PSVs) (PubMed:27288222). {ECO:0000269\|PubMed:16103374, ECO:0000269\|PubMed:23682115, ECO:0000269\|PubMed:24824487, ECO:0000269\|PubMed:27288222}.


Activity regulation:		Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. {ECO:0000305\|PubMed:24824487}.
Subunit:		Interacts with VPS9A homodimer (PubMed:18055610, PubMed:20833725). Interacts with TCTP1 (PubMed:20736351). Interacts with MON1 (PubMed:24824487). Interacts with EREX (via PX domain) (PubMed:27288222). Binds to VPS3 (PubMed:29463724). {ECO:0000269\|PubMed:18055610, ECO:0000269\|PubMed:20736351, ECO:0000269\|PubMed:20833725, ECO:0000269\|PubMed:24824487, ECO:0000269\|PubMed:27288222, ECO:0000269\|PubMed:29463724}.
Subcellular location:		Early endosome membrane {ECO:0000269\|PubMed:11532937, ECO:0000269\|PubMed:23482856}; Lipid- anchor {ECO:0000305}. Endosome membrane {ECO:0000269\|PubMed:15608333, ECO:0000269\|PubMed:16103374, ECO:0000269\|PubMed:24824487}; Lipid-anchor {ECO:0000305}. Prevacuolar compartment membrane {ECO:0000269\|PubMed:15509844, ECO:0000269\|PubMed:24824487}; Lipid- anchor {ECO:0000305}. Endosome, multivesicular body membrane {ECO:0000269\|PubMed:23682115}; Lipid-anchor {ECO:0000305}. Cell membrane {ECO:0000305\|PubMed:23519409}; Lipid-anchor {ECO:0000305}. Cytoplasm {ECO:0000269\|PubMed:29463724}. Note=Strong co-localization with VPS3 and VPS18 in cytoplasmic puncta. {ECO:0000269\|PubMed:29463724}.
Tissue specificity:		Expressed in roots and actively dividing cells. {ECO:0000269\|PubMed:11532937}.
Developmental stage:		Expressed during pollen germination and pollen tube growth. {ECO:0000269\|PubMed:18775970}.
Induction:		Activated by VPS9A. {ECO:0000269\|PubMed:18055610}.
Miscellaneous:		Over-expression of the constitutively active GTP-bound mutant of Leu-69 induces the formation of large ring-like structures of 1-2 micrometers in diameter. {ECO:0000269\|PubMed:23682115}.
Similarity:		Belongs to the small GTPase superfamily. Rab family. {ECO:0000305}.