PDBsum entry 2e43

Transcription/DNA

PDB id: 2e43

Contents

Protein chains

62 a.a. *

DNA/RNA

Waters ×331

* Residue conservation analysis

PDB id:

2e43

Name:

Transcription/DNA

Title:

Crystal structure of c/ebpbeta bzip homodimer k269a mutant bound to a high affinity DNA fragment

Structure:

DNA (5'- d(p Dtp Dap Dgp Dgp Dap Dtp Dtp Dgp Dcp Dgp Dcp Dap Dap Dtp Dap Dt)- 3'). Chain: c. Engineered: yes. DNA (5'- d(p Dap Dap Dtp Dap Dtp Dtp Dgp Dcp Dgp Dcp Dap Dap Dtp Dcp Dcp Dt)- 3'). Chain: d.

Source:

Synthetic: yes. Homo sapiens. Human. Organism_taxid: 9606. Gene: cebpb, tcf5. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

2.10Å R-factor: 0.222 R-free: 0.259

Authors:

T.H.Tahirov,T.Inoue-Bungo,K.Sato,M.Shiina,K.Hamada,K.Ogata

Key ref:

T.H.Tahirov et al. Structural basis for flexible base recognition by c/ebpbeta. To be published, .

Date:

01-Dec-06 Release date: 18-Dec-07

Protein chains

P17676  (CEBPB_HUMAN) -  CCAAT/enhancer-binding protein beta from Homo sapiens

Seq: 345 a.a.

Struc: 62 a.a.

DNA/RNA chains

T-A-G-G-A-T-T-G-C-G-C-A-A-T-A-T 16 bases

A-A-T-A-T-T-G-C-G-C-A-A-T-C-C-T 16 bases