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PDBsum entry 2dwz
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References listed in PDB file
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Key reference
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Title
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Structure of the oncoprotein gankyrin in complex with s6 atpase of the 26s proteasome.
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Authors
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Y.Nakamura,
K.Nakano,
T.Umehara,
M.Kimura,
Y.Hayashizaki,
A.Tanaka,
M.Horikoshi,
B.Padmanabhan,
S.Yokoyama.
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Ref.
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Structure, 2007,
15,
179-189.
[DOI no: ]
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PubMed id
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Note: In the PDB file this reference is
annotated as "TO BE PUBLISHED". The citation details given above have
been manually determined.
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Abstract
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Gankyrin is an oncoprotein commonly overexpressed in most hepatocellular
carcinomas. Gankyrin interacts with S6 ATPase of the 19S regulatory particle of
the 26S proteasome and enhances the degradation of the tumor suppressors pRb and
p53. Here, we report the structure of gankyrin in complex with the C-terminal
domain of S6 ATPase. Almost all of the seven ankyrin repeats of gankyrin
interact, through its concave region, with the C-terminal domain of S6 ATPase.
The intermolecular interactions occur through the complementary charged residues
between gankyrin and S6 ATPase. Biochemical studies based on the structure of
the complex revealed that gankyrin interacts with pRb in both the presence and
absence of S6 ATPase; however, the E182 residue in gankyrin is essential for the
pRb interaction. These results provide a structural basis for the involvement of
gankyrin in the pRb degradation pathway, through its association with S6 ATPase
of the 26S proteasome.
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Figure 2.
Figure 2. Structure of the Gankyrin/S6-C Complex
(A)
Ribbon representation of the mouse gankyrin/S6-C complex. Each
ankyrin repeat of the gankyrin is indicated by ANK1–ANK7 (blue
to red). The S6-C tertiary structure is colored orange. The N
and C termini of both molecules are indicated. Structural
figures were generated with PyMOL
(http://pymol.sourceforge.net/).
(B) van der Waals surface
representation of the protein complex. Gankyrin and S6-C are
shown in pink and orange, respectively.
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Figure 6.
Figure 6. Model of Gankyrin in Complex with the Complete
ATPase Domain of S6 ATPase
The structure of the
gankyrin/S6-C complex is superposed between the S6-C domain and
the C-terminal domain of the FtsH_E.coli structure for the
Cα-atoms. The coloring code for the gankyrin/S6-C complex is
the same as in Figure 2A, and the FtsH_E. coli structure is
shown in yellow.
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The above figures are
reprinted
by permission from Cell Press:
Structure
(2007,
15,
179-189)
copyright 2007.
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