PDBsum entry 2dw2

Apoptosis, toxin

PDB id

2dw2

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Contents

			Protein chains

					415 a.a. *

			Ligands

					NAG-NAG-BMA-MAN- NAG-MAN-NAG-NAG- FUC ×2

			Metals

					_CA ×6


					_ZN ×2

			Waters ×151

* Residue conservation analysis

PDB id:

2dw2

Links

Name:

Apoptosis, toxin

Title:

Crystal structure of vap2 from crotalus atrox venom (form 2-5 crystal)

Structure:

Catrocollastatin. Chain: a, b. Fragment: residues 191-609. Synonym: vap2, vascular apoptosis-inducing protein-2, catrocollastatin/vap2b

Source:

Crotalus atrox. Western diamondback rattlesnake. Organism_taxid: 8730

Resolution:

2.70Å

R-factor:

0.199

R-free:

0.260

Authors:

S.Takeda,T.Igarashi,S.Araki

Key ref:

T.Igarashi et al. (2007). Crystal structures of catrocollastatin/VAP2B reveal a dynamic, modular architecture of ADAM/adamalysin/reprolysin family proteins. Febs Lett, 581, 2416-2422. PubMed id: 17485084

Date:

02-Aug-06

Release date:

10-Jul-07

PROCHECK

	Headers

	References

Protein chains

Q90282 (VM3VB_CROAT) - Zinc metalloproteinase-disintegrin-like VAP2B from Crotalus atrox

Seq: Struc:

Seq: Struc:					609 a.a. 415 a.a.*

Key:

PfamA domain

Secondary structure

CATH domain

* PDB and UniProt seqs differ at 1 residue position (black cross)



		Enzyme reactions

Enzyme class:

E.C.3.4.24.- - ?????

[IntEnz] [ExPASy] [KEGG] [BRENDA]

	Febs Lett 581:2416-2422 (2007)
PubMed id: 17485084

Crystal structures of catrocollastatin/VAP2B reveal a dynamic, modular architecture of ADAM/adamalysin/reprolysin family proteins.
T.Igarashi, S.Araki, H.Mori, S.Takeda.

ABSTRACT

Catrocollastatin/vascular apoptosis-inducing protein (VAP)2B is a metalloproteinase from Crotalus atrox venom, possessing metalloproteinase/disintegrin/cysteine-rich (MDC) domains that bear the typical domain architecture of a disintegrin and metalloproteinase (ADAM)/adamalysin/reprolysin family proteins. Here we describe crystal structures of catrocollastatin/VAP2B in three different crystal forms, representing the first reported crystal structures of a member of the monomeric class of this family of proteins. The overall structures show good agreement with both monomers of atypical homodimeric VAP1. Comparison of the six catrocollastatin/VAP2B monomer structures and the structures of VAP1 reveals a dynamic, modular architecture that may be important for the functions of ADAM/adamalysin/reprolysin family proteins.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21333669

A.Pinyachat, P.Rojnuckarin, C.Muanpasitporn, P.Singhamatr, and S.Nuchprayoon (2011).
Albocollagenase, a novel recombinant P-III snake venom metalloproteinase from green pit viper (Cryptelytrops albolabris), digests collagen and inhibits platelet aggregation.

Toxicon, 57, 772-780.

21415364

C.J.Tape, S.H.Willems, S.L.Dombernowsky, P.L.Stanley, M.Fogarasi, W.Ouwehand, J.McCafferty, and G.Murphy (2011).
Cross-domain inhibition of TACE ectodomain.

Proc Natl Acad Sci U S A, 108, 5578-5583.

21255601

T.Teklemariam, A.I.Seoane, C.J.Ramos, E.E.Sanchez, S.E.Lucena, J.C.Perez, S.A.Mandal, and J.G.Soto (2011).
Functional analysis of a recombinant PIII-SVMP, GST-acocostatin; an apoptotic inducer of HUVEC and HeLa, but not SK-Mel-28 cells.

Toxicon, 57, 646-656.

19692335

H.Liu, A.H.Shim, and X.He (2009).
Structural characterization of the ectodomain of a disintegrin and metalloproteinase-22 (ADAM22), a neural adhesion receptor instead of metalloproteinase: insights on ADAM function.

J Biol Chem, 284, 29077-29086.

19643179

L.Troeberg, K.Fushimi, S.D.Scilabra, H.Nakamura, V.Dive, I.B.Thøgersen, J.J.Enghild, and H.Nagase (2009).
The C-terminal domains of ADAMTS-4 and ADAMTS-5 promote association with N-TIMP-3.

Matrix Biol, 28, 463-469.

18706512

S.Takeda (2009).
Three-dimensional domain architecture of the ADAM family proteinases.

Semin Cell Dev Biol, 20, 146-152.

18344021

A.Zolkiewska (2008).
ADAM proteases: ligand processing and modulation of the Notch pathway.

Cell Mol Life Sci, 65, 2056-2068.

18479462

J.W.Fox, and S.M.Serrano (2008).
Insights into and speculations about snake venom metalloproteinase (SVMP) synthesis, folding and disulfide bond formation and their contribution to venom complexity.

FEBS J, 275, 3016-3030.

18391413

N.Moiseeva, R.Bau, S.D.Swenson, F.S.Markland, J.Y.Choe, Z.J.Liu, and M.Allaire (2008).
Structure of acostatin, a dimeric disintegrin from Southern copperhead (Agkistrodon contortrix contortrix), at 1.7 A resolution.

Acta Crystallogr D Biol Crystallogr, 64, 466-470.

PDB code:

3c05

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.