PDBsum entry 2do6

Ligase

PDB id: 2do6

Contents

Protein chains

53 a.a. *

* Residue conservation analysis

PDB id:

2do6

Name:

Ligase

Title:

Solution structure of rsgi ruh-065, a uba domain from human cdna

Structure:

E3 ubiquitin-protein ligase cbl-b. Chain: a, b. Fragment: uba domain. Synonym: signal transduction protein cbl-b, sh3-binding protein cbl- b, casitas b-lineage lymphoma proto-oncogene b, ring finger protein 56, rsgi ruh-065. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: cblb, rnf56. Other_details: cell-free protein synthesis

NMR struc:

20 models

Authors:

T.Hamada,H.Hirota,Y.-J.Lin,P.Guntert,M.Sato,S.Koshiba,T.Kigawa, S.Yokoyama,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

T.Hamada et al. Solution structure of rsgi ruh-065, A uba domain from human cdna. To be published, .

Date:

27-Apr-06 Release date: 08-May-07

Protein chains

Q13191  (CBLB_HUMAN) -  E3 ubiquitin-protein ligase CBL-B from Homo sapiens

Seq: 982 a.a.

Struc: 53 a.a.*

* PDB and UniProt seqs differ at 11 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine