PDBsum entry 2dmv

Ligase

PDB id: 2dmv

Contents

Protein chain

43 a.a. *

* Residue conservation analysis

PDB id:

2dmv

Name:

Ligase

Title:

Solution structure of the second ww domain of itchy homolog e3 ubiquitin protein ligase (itch)

Structure:

Itchy homolog e3 ubiquitin protein ligase. Chain: a. Fragment: ww domain. Synonym: itch, atrophin-1-interacting protein 4, aip4, nfe2- associated polypeptide 1, napp1. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: itch. Expressed in: cell free synthesis. Other_details: cell-free protein synthesis

NMR struc:

20 models

Authors:

S.Ohnishi,K.Paakkonen,N.Tochio,T.Tomizawa,S.Koshiba,M.Inoue, P.Guntert,T.Kigawa,S.Yokoyama,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

S.Ohnishi et al. Solution structure of the second ww domain of itchy homolog e3 ubiquitin protein ligase (itch). To be published, .

Date:

24-Apr-06 Release date: 24-Oct-06

Protein chain

Q96J02  (ITCH_HUMAN) -  E3 ubiquitin-protein ligase Itchy homolog from Homo sapiens

Seq: 903 a.a.

Struc: 43 a.a.*

* PDB and UniProt seqs differ at 12 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine