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PDBsum entry 2dln
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Ligase(peptidoglycan synthesis)
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PDB id
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2dln
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References listed in PDB file
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Key reference
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Title
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Vancomycin resistance: structure of d-Alanine:d-Alanine ligase at 2.3 a resolution.
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Authors
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C.Fan,
P.C.Moews,
C.T.Walsh,
J.R.Knox.
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Ref.
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Science, 1994,
266,
439-443.
[DOI no: ]
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PubMed id
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Abstract
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The molecular structure of the D-alanine:D-alanine ligase of the ddlB gene of
Escherichia coli, co-crystallized with an S,R-methylphosphinate and adenosine
triphosphate, was determined by x-ray diffraction to a resolution of 2.3
angstroms. A catalytic mechanism for the ligation of two D-alanine substrates is
proposed in which a helix dipole and a hydrogen-bonded triad of tyrosine,
serine, and glutamic acid assist binding and deprotonation steps. From sequence
comparison, it is proposed that a different triad exists in a recently
discovered D-alanine:D-lactate ligase (VanA) present in vancomycin-resistant
enterococci. A molecular mechanism for the altered specificity of VanA is
suggested.
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