PDBsum entry 2dja

Metal binding protein

PDB id: 2dja

Contents

Protein chain

84 a.a. *

Metals

_ZN ×2

* Residue conservation analysis

PDB id:

2dja

Name:

Metal binding protein

Title:

Solution structure of the b-box domain of the human midline-2 protein

Structure:

Midline-2. Chain: a. Fragment: zf-b_box. Synonym: midline defect 2, tripartite motif protein 1, midin-2, ring finger protein 60. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: mid2, fxy2, rnf60, trim1. Expressed in: cell free synthesis. Other_details: cell-free protein synthesis

NMR struc:

20 models

Authors:

K.Miyamoto,T.Kigawa,T.Tomizawa,S.Koshiba,M.Inoue,S.Yokoyama,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

K.Miyamoto et al. Solution structure of the b-Box domain of the human midline-2 protein. To be published, .

Date:

31-Mar-06 Release date: 01-Oct-06

Protein chain

Q9UJV3  (TRIM1_HUMAN) -  Probable E3 ubiquitin-protein ligase MID2 from Homo sapiens

Seq: 735 a.a.

Struc: 84 a.a.*

* PDB and UniProt seqs differ at 13 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.3.2.27 - RING-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine