PDBsum entry 2dag

Hydrolase

PDB id: 2dag

Contents

Protein chain

74 a.a. *

* Residue conservation analysis

PDB id:

2dag

Name:

Hydrolase

Title:

Solution structure of the first uba domain in the human ubiquitin specific protease 5 (isopeptidase 5)

Structure:

Ubiquitin carboxyl-terminal hydrolase 5. Chain: a. Fragment: uba domain. Synonym: ubiquitin thiolesterase 5, ubiquitin-specific processing protease 5, deubiquitinating enzyme 5, isopeptidase t. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: usp5. Expressed in: cell free synthesis. Other_details: cell-free protein synthesis

NMR struc:

20 models

Authors:

C.Zhao,T.Kigawa,T.Tomizawa,S.Koshiba,M.Inoue,S.Yokoyama,Riken Structural Genomics/proteomics Initiative (Rsgi)

Key ref:

C.Zhao et al. Solution structure of the first uba domain in the human ubiquitin specific protease 5 (isopeptidase 5). To be published, .

Date:

14-Dec-05 Release date: 14-Jun-06

Protein chain

P45974  (UBP5_HUMAN) -  Ubiquitin carboxyl-terminal hydrolase 5 from Homo sapiens

Seq: 858 a.a.

Struc: 74 a.a.*

* PDB and UniProt seqs differ at 11 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.4.19.12 - ubiquitinyl hydrolase 1.
• Reaction: Thiol-dependent hydrolysis of ester, thiolester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).