UniProt functional annotation for O75154



	UniProt functional annotation for O75154

UniProt code: O75154.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Acts as a regulator of endocytic traffic by participating in membrane delivery. Required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis. Also required for the structural integrity of the endosomal recycling compartment during interphase. May play a role in breast cancer cell motility by regulating actin cytoskeleton. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non- processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494). {ECO:0000269|PubMed:15601896, ECO:0000269|PubMed:17394487, ECO:0000269|PubMed:19327867, ECO:0000269|PubMed:25035494}.

Subunit: Homodimer. Forms a complex with Rab11 (RAB11A or RAB11B) and ARF6. Interacts with RAB11A; the interaction is direct. Interacts with RAB11B, RAB25 and RAB11FIP4. Interacts with ARF6; according to PubMed:16148947, it specifically interacts with ARF6 but not ARF5. Interacts with ARF6; according to PubMed:17030804 but not PubMed:16148947. Interacts with RACGAP1/MgcRacGAP; interaction takes place during late stage of cytokinesis and is required for recruitment to the midbody. Interacts with ASAP1 and EXOC7. Interacts with dynein intermediate chain and dynactin (DCTN1) (PubMed:25035494). {ECO:0000269|PubMed:11495908, ECO:0000269|PubMed:12470645, ECO:0000269|PubMed:15601896, ECO:0000269|PubMed:16148947, ECO:0000269|PubMed:17007872, ECO:0000269|PubMed:17030804, ECO:0000269|PubMed:17229837, ECO:0000269|PubMed:17628206, ECO:0000269|PubMed:18511905, ECO:0000269|PubMed:25035494}.

Subcellular location: Recycling endosome membrane {ECO:0000269|PubMed:11495908, ECO:0000269|PubMed:15601896, ECO:0000269|PubMed:17007872, ECO:0000269|PubMed:17394487}; Peripheral membrane protein. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269|PubMed:15158446, ECO:0000269|PubMed:15601896, ECO:0000269|PubMed:17394487, ECO:0000269|PubMed:18511905}. Cleavage furrow {ECO:0000269|PubMed:15158446, ECO:0000269|PubMed:15601896}. Midbody {ECO:0000269|PubMed:15601896, ECO:0000269|PubMed:16148947, ECO:0000269|PubMed:18511905}. Note=In early mitosis remains diffuse and distributed through the cell. The onset of anaphase sequesters these vesicles to the centrosomes at the opposite poles of the cell. During telophase these vesicles move from the centrosomes, to the furrow, and then to the midbody to aid in abscission (PubMed:15158446, PubMed:15601896, PubMed:18511905). Interaction with Rab11 mediates localization to endosomes (PubMed:11495908). Interaction with ARF6 mediates localization to the midbody (PubMed:16148947). {ECO:0000269|PubMed:11495908, ECO:0000269|PubMed:15601896, ECO:0000269|PubMed:16148947, ECO:0000269|PubMed:18511905}.

Domain: The RBD-FIP domain mediates the interaction with Rab11 (RAB11A or RAB11B). {ECO:0000269|PubMed:17030804}.

Ptm: Phosphorylated at Ser-102 by CDK1 during metaphase, and dephosphorylated as cells enter telophase. {ECO:0000269|PubMed:22401586}.

Sequence caution: Sequence=BAA31640.2; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAG57098.1; Type=Erroneous initiation; Evidence={ECO:0000305};

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Acts as a regulator of endocytic traffic by participating in membrane delivery. Required for the abcission step in cytokinesis, possibly by acting as an 'address tag' delivering recycling endosome membranes to the cleavage furrow during late cytokinesis. Also required for the structural integrity of the endosomal recycling compartment during interphase. May play a role in breast cancer cell motility by regulating actin cytoskeleton. Acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non- processive to a highly processive motor in the presence of dynactin. Facilitates the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track) (PubMed:25035494). {ECO:0000269\|PubMed:15601896, ECO:0000269\|PubMed:17394487, ECO:0000269\|PubMed:19327867, ECO:0000269\|PubMed:25035494}.


Subunit:		Homodimer. Forms a complex with Rab11 (RAB11A or RAB11B) and ARF6. Interacts with RAB11A; the interaction is direct. Interacts with RAB11B, RAB25 and RAB11FIP4. Interacts with ARF6; according to PubMed:16148947, it specifically interacts with ARF6 but not ARF5. Interacts with ARF6; according to PubMed:17030804 but not PubMed:16148947. Interacts with RACGAP1/MgcRacGAP; interaction takes place during late stage of cytokinesis and is required for recruitment to the midbody. Interacts with ASAP1 and EXOC7. Interacts with dynein intermediate chain and dynactin (DCTN1) (PubMed:25035494). {ECO:0000269\|PubMed:11495908, ECO:0000269\|PubMed:12470645, ECO:0000269\|PubMed:15601896, ECO:0000269\|PubMed:16148947, ECO:0000269\|PubMed:17007872, ECO:0000269\|PubMed:17030804, ECO:0000269\|PubMed:17229837, ECO:0000269\|PubMed:17628206, ECO:0000269\|PubMed:18511905, ECO:0000269\|PubMed:25035494}.
Subcellular location:		Recycling endosome membrane {ECO:0000269\|PubMed:11495908, ECO:0000269\|PubMed:15601896, ECO:0000269\|PubMed:17007872, ECO:0000269\|PubMed:17394487}; Peripheral membrane protein. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome {ECO:0000269\|PubMed:15158446, ECO:0000269\|PubMed:15601896, ECO:0000269\|PubMed:17394487, ECO:0000269\|PubMed:18511905}. Cleavage furrow {ECO:0000269\|PubMed:15158446, ECO:0000269\|PubMed:15601896}. Midbody {ECO:0000269\|PubMed:15601896, ECO:0000269\|PubMed:16148947, ECO:0000269\|PubMed:18511905}. Note=In early mitosis remains diffuse and distributed through the cell. The onset of anaphase sequesters these vesicles to the centrosomes at the opposite poles of the cell. During telophase these vesicles move from the centrosomes, to the furrow, and then to the midbody to aid in abscission (PubMed:15158446, PubMed:15601896, PubMed:18511905). Interaction with Rab11 mediates localization to endosomes (PubMed:11495908). Interaction with ARF6 mediates localization to the midbody (PubMed:16148947). {ECO:0000269\|PubMed:11495908, ECO:0000269\|PubMed:15601896, ECO:0000269\|PubMed:16148947, ECO:0000269\|PubMed:18511905}.
Domain:		The RBD-FIP domain mediates the interaction with Rab11 (RAB11A or RAB11B). {ECO:0000269\|PubMed:17030804}.
Ptm:		Phosphorylated at Ser-102 by CDK1 during metaphase, and dephosphorylated as cells enter telophase. {ECO:0000269\|PubMed:22401586}.
Sequence caution:		Sequence=BAA31640.2; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAG57098.1; Type=Erroneous initiation; Evidence={ECO:0000305};