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PDBsum entry 2d7c
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Protein transport
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PDB id
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2d7c
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References listed in PDB file
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Key reference
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Title
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Structural basis for rab11-Dependent membrane recruitment of a family of rab11-Interacting protein 3 (fip3)/arfophilin-1.
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Authors
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T.Shiba,
H.Koga,
H.W.Shin,
M.Kawasaki,
R.Kato,
K.Nakayama,
S.Wakatsuki.
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Ref.
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Proc Natl Acad Sci U S A, 2006,
103,
15416-15421.
[DOI no: ]
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PubMed id
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Note In the PDB file this reference is
annotated as "TO BE PUBLISHED".
The citation details given above were identified by an automated
search of PubMed on title and author
names, giving a
percentage match of
95%.
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Abstract
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Family of Rab11-interacting protein (FIP)3/Arfophlin-1 and FIP4/Arfophilin-2 are
dual effectors for Rab11 and ADP ribosylation factor (ARF)5/ARF6, which are
involved in membrane delivery from recycling endosomes to the plasma membrane
during cytokinesis. Here, we define the distinct C-terminal binding regions of
FIP3 and FIP4 for Rab11 and ARF5/ARF6. Furthermore, we determined the crystal
structure of Rab11 in complex with the Rab11-binding domain (RBD) of FIP3. The
long amphiphilic alpha-helix of FIP3-RBD forms a parallel coiled-coil homodimer,
with two symmetric interfaces with two Rab11 molecules. The hydrophobic side of
the RBD helix is involved in homodimerization and mediates the interaction with
the Rab11 switch 1 region, whereas the opposite hydrophilic side interacts with
the Rab11 switch 2 and is the major factor contributing to the binding
specificity. The bivalent interaction of FIP3 with Rab11 at the C terminus
allows FIP3 to coordinately function with other binding partners, including ARFs.
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Figure 4.
Fig. 4. Superposition of Rab11 in different forms. The
switch 1 and switch 2 regions are shown. The side chains of
Arg-72 and Arg-74 in the switch 2 region are indicated by
ball-and-stick models. GTP/GDP molecules also are presented as
ball-and-stick models. The GDP-bound form (PDB ID code 1OIV) is
blue, the GTP-bound free form (PDB ID code 1OIW) is green, and
molecule A GTP-bound Rab11 in complex with FIP3-RBD is red,
whereas molecule B is orange.
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Figure 6.
Fig. 6. Interaction between Rab11 and FIP3-RBD. Shown are
close-up views of the switch 1 (a), switch 2 (b), and
interswitch (c) regions of Rab11 in complex with FIP3-RBD. The
color scheme is the same as that of Fig. 3b. Residues involved
in the interaction are labeled and indicated by ball-and-stick
models. The cyan dashed lines indicate hydrogen bonds or
electrostatic interactions.
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