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PDBsum entry 2by6
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References listed in PDB file
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Key reference
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Title
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Is radiation damage dependent on the dose rate used during macromolecular crystallography data collection?
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Authors
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H.K.Leiros,
J.Timmins,
R.B.Ravelli,
S.M.Mcsweeney.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2006,
62,
125-132.
[DOI no: ]
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PubMed id
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Abstract
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This paper focuses on the radiation-damage effects when applying the same total
X-ray dose to protein crystals at different dose rates. These experiments have
been performed on both a selenomethionated protein and on bovine trypsin using
dose rates that span nearly two orders of magnitude. The results show no clear
dose-rate effect on the global indicators of radiation damage, but a small
measurable dose-rate effect could be found when studying specific radiation
damage. It is hypothesized that this observed dose-rate effect relates to
differences in the steady-state free-radical concentration.
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Figure 2.
Figure 2
(a) Illustrations of the disulfide bond from Cys395 to the Bme residue and (b) the seven
SeMet residues (Mse), with the corresponding vector difference map from the drMTH-Rate75
burn displayed at +4 [sigma] (red) and -4 [sigma] (green).
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Figure 5.
Figure 5
Active site of BT: an illustration of the binding pocket in BT with the vector difference
map from BT-Rate24 contoured at +4 [sigma] (red) and -4 [sigma] (green). The
inhibitor benzamidine (Bza), Asp189, Ser190, Tyr228 and some surrounding water molecules
are shown.
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The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(2006,
62,
125-132)
copyright 2006.
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