 |
PDBsum entry 2brs
|
|
|
|
References listed in PDB file
|
 |
|
Key reference
|
|
|
Title
|
|
Eosinophil-Granule major basic protein, A c-Type lectin, Binds heparin.
|
|
|
Authors
|
|
G.J.Swaminathan,
D.G.Myszka,
P.S.Katsamba,
L.E.Ohnuki,
G.J.Gleich,
K.R.Acharya.
|
|
|
Ref.
|
|
Biochemistry, 2005,
44,
14152-14158.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
The eosinophil major basic protein (EMBP), a constituent of the eosinophil
secondary granule, is implicated in cytotoxicity and mediation of allergic
disorders such as asthma. It is a member of the C-type lectin family, but lacks
a Ca(2+)- and carbohydrate-binding site as seen in other members of this family.
Here, we report the crystal structure of EMBP in complex with a heparin
disaccharide and in the absence of Ca(2+), the first such report of any C-lectin
with this sugar. We also provide direct evidence of binding of EMBP to heparin
and heparin disaccharide by surface plasmon resonance. We propose that the
sugars recognized by EMBP are likely to be proteoglycans such as heparin,
leading to new interpretations for EMBP function.
|
|
|
|
|
|
|
