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PDBsum entry 2bpc
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Nucleotidyltransferase
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PDB id
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2bpc
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References listed in PDB file
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Key reference
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Title
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Crystal structure of rat DNA polymerase beta: evidence for a common polymerase mechanism.
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Authors
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M.R.Sawaya,
H.Pelletier,
A.Kumar,
S.H.Wilson,
J.Kraut.
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Ref.
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Science, 1994,
264,
1930-1935.
[DOI no: ]
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PubMed id
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Abstract
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Structures of the 31-kilodalton catalytic domain of rat DNA polymerase beta (pol
beta) and the whole 39-kilodalton enzyme were determined at 2.3 and 3.6 angstrom
resolution, respectively. The 31-kilodalton domain is composed of fingers, palm,
and thumb subdomains arranged to form a DNA binding channel reminiscent of the
polymerase domains of the Klenow fragment of Escherichia coli DNA polymerase I,
HIV-1 reverse transcriptase, and bacteriophage T7 RNA polymerase. The
amino-terminal 8-kilodalton domain is attached to the fingers subdomain by a
flexible hinge. The two invariant aspartates found in all polymerase sequences
and implicated in catalytic activity have the same geometric arrangement within
structurally similar but topologically distinct palms, indicating that the
polymerases have maintained, or possibly re-evolved, a common nucleotidyl
transfer mechanism. The location of Mn2+ and deoxyadenosine triphosphate in pol
beta confirms the role of the invariant aspartates in metal ion and
deoxynucleoside triphosphate binding.
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Secondary reference #1
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Title
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Structures of ternary complexes of rat DNA polymerase beta, A DNA template-Primer, And ddctp.
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Authors
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H.Pelletier,
M.R.Sawaya,
A.Kumar,
S.H.Wilson,
J.Kraut.
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Ref.
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Science, 1994,
264,
1891-1903.
[DOI no: ]
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PubMed id
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Secondary reference #2
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Title
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Studies of the domain structure of mammalian DNA polymerase beta. Identification of a discrete template binding domain.
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Authors
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A.Kumar,
S.G.Widen,
K.R.Williams,
P.Kedar,
R.L.Karpel,
S.H.Wilson.
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Ref.
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J Biol Chem, 1990,
265,
2124-2131.
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PubMed id
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