 |
PDBsum entry 2bou
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Immune system
|
PDB id
|
|
|
|
2bou
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
References listed in PDB file
|
|
|
Key reference
|
|
|
Title
|
|
Structural and functional characterization of a novel t cell receptor co-Regulatory protein complex, Cd97-Cd55.
|
|
|
Authors
|
|
R.J.Abbott,
I.Spendlove,
P.Roversi,
H.Fitzgibbon,
V.Knott,
P.Teriete,
J.M.Mcdonnell,
P.A.Handford,
S.M.Lea.
|
|
|
Ref.
|
|
J Biol Chem, 2007,
282,
22023-22032.
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
CD97, the archetypal member of the EGF-TM7 protein family, is constitutively
expressed on granulocytes and monocytes and rapidly up-regulated on T and B
cells following activation. The key isoform of CD97 expressed on leukocytes
binds the complement regulatory protein CD55 (also termed decay-accelerating
factor). CD97 has been shown recently to mediate co-stimulation of T cells via
CD55. Here, we demonstrate that blocking the interaction between CD55 on
monocytes and CD97 on T cells leads to inhibition of proliferation and
interferon-gamma secretion. This implies that bidirectional interactions between
CD97 and CD55 are involved in T cell regulation. Structural studies presented
here reveal the molecular basis for this activity. We have solved the structure
of EMR2, a very close homolog of CD97, using x-ray crystallography. NMR-based
chemical shift mapping of the EMR2-CD55 interaction has allowed us to generate a
model for the CD97-CD55 complex. The structure of the complex reveals that the T
cell and complement regulatory activities of CD55 occur on opposite faces of the
molecule. This suggests that CD55 might simultaneously regulate both the innate
and adaptive immune responses, and we have shown that CD55 can still regulate
complement when bound to CD97.
|
|
|
Secondary reference #1
|
|
|
Title
|
|
Crystallization and preliminary X-Ray diffraction analysis of three egf domains of emr2, A 7tm immune-System molecule.
|
|
|
Authors
|
|
R.J.Abbott,
V.Knott,
P.Roversi,
S.Neudeck,
P.Lukacik,
P.A.Handford,
S.M.Lea.
|
|
|
Ref.
|
|
Acta Crystallogr D Biol Crystallogr, 2004,
60,
936-938.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
|
|
|
Figure 1.
Figure 1 Ba^2+ crystals of EGF domains 1, 2 and 5 from EMR2
(300 µm in the longest direction).
|
|
|
|
|
|
The above figure is
reproduced from the cited reference
with permission from the IUCr
|
|
|
|
|
|
|
