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UniProt functional annotation for Q6DE08 | ||
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| UniProt code: Q6DE08. |
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| Organism: | |
Xenopus laevis (African clawed frog). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia; Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus. |
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| Function: | |
Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage. Phosphorylates 'Ser-10' of histone H3 during mitosis. {ECO:0000269|PubMed:11350965, ECO:0000269|PubMed:12221116, ECO:0000269|PubMed:17199039}. |
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| Catalytic activity: | |
Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl- [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA- COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence={ECO:0000269|PubMed:11350965, ECO:0000269|PubMed:12221116}; |
| Catalytic activity: | |
Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L- threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1; Evidence={ECO:0000269|PubMed:11350965, ECO:0000269|PubMed:12221116}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000250}; |
| Activity regulation: | |
Kinase activity is stimulated by both birc5/survivin-binding and cell-cycle specific phosphorylation. {ECO:0000269|PubMed:11350965, ECO:0000269|PubMed:12221116}. |
| Subunit: | |
Component of the CPC at least composed of survivin/birc5, incenp, cdca8/borealin and/or cdca9/dasra-A, and aurkb/aurora-B. Interacts directly (via N-terminus and kinase domain) with incenp (via C terminus), and may weakly interact (via N-terminus) with birc5.1 to stabilize the complex. Interacts with mtus1. {ECO:0000269|PubMed:10996078, ECO:0000269|PubMed:12221116, ECO:0000269|PubMed:12464631, ECO:0000269|PubMed:12919681, ECO:0000269|PubMed:15260989, ECO:0000269|PubMed:15866179, ECO:0000269|PubMed:16322459, ECO:0000269|PubMed:17199039}. |
| Subcellular location: | |
Nucleus. Chromosome. Note=Chromosomal until metaphase but transfers to the midzone microtubule array and the equatorial cortex during anaphase. |
| Ptm: | |
Phosphorylated, stimulates kinase activity. {ECO:0000269|PubMed:12221116}. |
| Similarity: | |
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. Aurora subfamily. {ECO:0000255|PROSITE- ProRule:PRU00159}. |
| Sequence caution: | |
Sequence=AAH41288.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305}; |
Annotations taken from UniProtKB at the EBI.