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PDBsum entry 2b9h
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References listed in PDB file
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Key reference
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Title
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The role of docking interactions in mediating signaling input, Output, And discrimination in the yeast mapk network.
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Authors
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A.Reményi,
M.C.Good,
R.P.Bhattacharyya,
W.A.Lim.
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Ref.
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Mol Cell, 2005,
20,
951-962.
[DOI no: ]
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PubMed id
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Abstract
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Cells use a network of mitogen-activated protein kinases (MAPKs) to coordinate
responses to diverse extracellular signals. Here, we examine the role of docking
interactions in determining connectivity of the yeast MAPKs Fus3 and Kss1. These
closely related kinases are activated by the common upstream MAPK kinase Ste7
yet generate distinct output responses, mating and filamentous growth,
respectively. We find that docking interactions are necessary for communication
with the kinases and that they can encode subtle differences in pathway-specific
input and output. The cell cycle arrest mediator Far1, a mating-specific
substrate, has a docking motif that selectively binds Fus3. In contrast, the
shared partner Ste7 has a promiscuous motif that binds both Fus3 and Kss1.
Structural analysis reveals that Fus3 interacts with specific and promiscuous
peptides in conformationally distinct modes. Induced fit recognition may allow
docking peptides to achieve discrimination by exploiting subtle differences in
kinase flexibility.
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Figure 1.
Figure 1. Docking Motifs Found in Interaction Partners of
the Saccharomyces cerevisiae MAPKs Fus3 and Kss1
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Figure 3.
Figure 3. Structure of the Fus3 MAPK
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The above figures are
reprinted
by permission from Cell Press:
Mol Cell
(2005,
20,
951-962)
copyright 2005.
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