PDBsum entry 2b8k

Transferase

PDB id: 2b8k

Contents

Protein chains

1416 a.a. *

1114 a.a. *

266 a.a. *

182 a.a. *

214 a.a. *

84 a.a. *

171 a.a. *

133 a.a. *

119 a.a. *

65 a.a. *

115 a.a. *

46 a.a. *

Metals

_ZN ×8

* Residue conservation analysis

PDB id:

2b8k

Name:

Transferase

Title:

12-subunit RNA polymerase ii

Structure:

DNA-directed RNA polymerase ii largest subunit. Chain: a. Synonym: RNA polymerase ii subunit 1, b220. DNA-directed RNA polymerase ii 140 kda polypeptide. Chain: b. Synonym: b150, RNA polymerase ii subunit 2. DNA-directed RNA polymerase ii 45 kda polypeptide. Chain: c. Synonym: b44.5.

Source:

Saccharomyces cerevisiae. Baker's yeast. Organism_taxid: 4932. Organism_taxid: 4932

Biol. unit:

Dodecamer (from PQS)

Resolution:

4.15Å R-factor: 0.387

Authors:

P.A.Meyer,P.Ye,M.Zhang,M.H.Suh,J.Fu

Key ref:

P.A.Meyer et al. (2006). Phasing RNA polymerase II using intrinsically bound Zn atoms: an updated structural model. Structure, 14, 973-982. PubMed id: 16765890 DOI: 10.1016/j.str.2006.04.003

Date:

07-Oct-05 Release date: 20-Jun-06

Protein chain

P04050  (RPB1_YEAST) -  DNA-directed RNA polymerase II subunit RPB1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1733 a.a.

Struc: 1416 a.a.

Protein chain

P08518  (RPB2_YEAST) -  DNA-directed RNA polymerase II subunit RPB2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 1224 a.a.

Struc: 1114 a.a.

Protein chain

P16370  (RPB3_YEAST) -  DNA-directed RNA polymerase II subunit RPB3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 318 a.a.

Struc: 266 a.a.

Protein chain

P20433  (RPB4_YEAST) -  DNA-directed RNA polymerase II subunit RPB4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 221 a.a.

Struc: 182 a.a.

Protein chain

P20434  (RPAB1_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC1 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 215 a.a.

Struc: 214 a.a.

Protein chain

P20435  (RPAB2_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC2 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 155 a.a.

Struc: 84 a.a.

Protein chain

P34087  (RPB7_YEAST) -  DNA-directed RNA polymerase II subunit RPB7 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 171 a.a.

Struc: 171 a.a.

Protein chain

P20436  (RPAB3_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC3 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 146 a.a.

Struc: 133 a.a.

Protein chain

P27999  (RPB9_YEAST) -  DNA-directed RNA polymerase II subunit RPB9 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 122 a.a.

Struc: 119 a.a.

Protein chain

P22139  (RPAB5_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 70 a.a.

Struc: 65 a.a.

Protein chain

P38902  (RPB11_YEAST) -  DNA-directed RNA polymerase II subunit RPB11 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 120 a.a.

Struc: 115 a.a.

Protein chain

P40422  (RPAB4_YEAST) -  DNA-directed RNA polymerases I, II, and III subunit RPABC4 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 70 a.a.

Struc: 46 a.a.

Enzyme reactions

• Enzyme class: Chains A, B, C, D, E, F, G, H, I, J, K, L: E.C.2.7.7.6 - DNA-directed Rna polymerase.
• Reaction: RNA(n) + a ribonucleoside 5'-triphosphate = RNA(n+1) + diphosphate

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

Added reference

DOI no: 10.1016/j.str.2006.04.003

Structure 14:973-982 (2006)

PubMed id: 16765890

Phasing RNA polymerase II using intrinsically bound Zn atoms: an updated structural model.

P.A.Meyer, P.Ye, M.Zhang, M.H.Suh, J.Fu.

ABSTRACT

Macromolecular assemblies as large as RNA polymerase II (Pol II) can be phased by a few intrinsically bound Zn atoms, by using MAD experiments as described here. A phasing effectiveness of 570 aa/Zn is attained for Pol II. The resulting experimental, unbiased electron density map is of such quality that it confirms the existing crystallographic model and further reveals structural regions not shown by model phases, thus updating the Pol II model at three sites. The mechanistically important fork loop-1 element is observed to be ordered in the absence of nucleic acids, suggesting additional insights into the mechanisms that maintain the stability of the transcription ternary complex and allow its release. Furthermore, a computational experiment with simulated MAD data sets demonstrates that 1 Zn site is able to provide adequate experimental phase information for as many as 1100 amino acids of polypeptide, under the conditions of the current synchrotron and detector technologies.

Selected figure(s)

Figure 1.
Figure 1. Calculating an Experimental Electron Density Map of Pol II by using Zn-MAD
(A) A representative anomalous difference Fourier map (7.2 Å) based on a set of Zn peak data and the model phases. Density contours are displayed at the 3.0σ (standard deviation) level in red. Three of the eight Zn sites are shown as green spheres.
(B) A representative dispersive difference Fourier map (7.2 Å) based on a set of dispersive data and the model phases. The same map parameters as in (A) were used in the calculation. The color scheme remains the same as in (A).
(C) A representative crosscrystal dispersive difference Fourier map (7.2 Å) used to assess crystal isomorphism. The map was calculated by using the model phases and amplitude differences between the remote data of crystal-1 and the inflection data of crystal-2. The map parameters and color scheme are the same as in (B).
(D–I) (D) An experimental map based solely on Zn anomalous peak data (SAS) and the known coordinates of the Zn sites. The map was calculated to 7.2 Å and is shown in red at 1.0σ. An existing 12 subunit Pol II model (Cα only) is shown in blue. The green wire model is a lattice-packing neighbor. The heterodimer of Rpb4/7 (indicated by “Rpb4/7”) protrudes to the right from the bottom of the clamp domain (indicated by “Clamp”). All of the maps in (D)–(I) were solvent flattened as described in Experimental Procedures. Map slab thickness, box sizes, and the color scheme are the same in (D)–(I). (E) An experimental map calculated from a Zn dispersive data set, treated as SIR. The resolution cutoff is the same as in (D). (F) A Zn-MAD map (7.2 Å) resulted from combining the anomalous and dispersive information. Lattice contacts (indicated by “Contacts”) clarify in the full MAD map, as indicated by the arrow. (G) The Zn-MAD map (7.2 Å) produced from combining multiple Zn-MAD phasing sets. The Rpb8 β sheet (indicated by “Rpb8”) is labeled. (H) The Zn-MAD map resulting from the manually edited mask (for solvent flattening). The polymerase active site is indicated by the arrow. (I) The final Zn-MAD map calculated to 4.1 Å. The map was computed with smaller grid spacing than the low-resolution maps described in other panels; thus, it appears more crowded. Significantly more details of the polypeptide chains become visible in this map. The arrow indicates the density for fork loop-1 that was disordered in previous uncomplexed Pol II structures.

Figure 2.
Figure 2. Updating the Pol II Model According to the Zn-MAD Densities
(A–C) (A) Definition of the loop structure at the tip of a two-helix stalk in Rpb2. Poly-alanines (green, Cα only) were fitted into the density, connecting the helices. Electron densities (red) are shown all at 1.0σ level for (A)–(C). The existing model of Pol II (Cα only) is colored blue in (A)–(C). Some of the side chain densities that projected off the α carbons were evident in the 4.1 Å Zn-MAD map. (B) The model for Rpb4 was updated according to the Zn-MAD densities. Residues 118–126 were adjusted to fit the density. Residues 113–117 were inserted according to the density. α carbons of the updated residues are shown in green. Again, partial densities of certain side groups protrude from the α carbons. (C) Localization of fork loop-1 (green, Cα model) according to its Zn-MAD density. Clear densities occurred at a site opposite the rudder over the cleft, and they could only be accounted for by fitting with fork loop-1. As shown by the touching densities, fork loop-1 contacts the rudder to form a set of “arms” that surrounds the DNA/RNA hybrid in the ternary complex as has been noted (Westover et al., 2004b). Residues involved in the contact are indicated. The bridge helix defining the floor of the cleft is indicated as well.
(D and E) (D) A motion by fork loop-1 can be seen from its different locations in the free Pol II (red, Cα model) and in ternary complexes (yellow and purple). The view is roughly from the downstream side through the cleft and against the wall (not visible here) of the hybrid tunnel. The rudder is shown in cyan, and the rest of Pol II is shown in blue. The DNA template strand is shown by the gray stick model, while the transcript RNA is indicated by the pink sticks. The downstream DNA (gray sticks) is roughly perpendicular to the plane of the paper. The dashed line indicates a cut-away plane against which is the viewing direction for (E). (E) Compartmentalization of the Pol II cleft by the protein mass of fork loop-1 (red) and the rudder (white). This is a cut-away view from the plane, as indicated in (D), against the clamp domain of Rpb1. The surface rendering shows the spatial arrangement of the hybrid tunnel and the downstream DNA channel.

The above figures are reprinted by permission from Cell Press: Structure (2006, 14, 973-982) copyright 2006.