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UniProt functional annotation for Q86MV5 | ||
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| UniProt code: Q86MV5. |
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| Organism: | |
Trypanosoma brucei brucei. |
| Taxonomy: | |
Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Trypanosoma. |
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| Function: | |
Terminal uridylyltransferase which, as part of the mitochondrial RNA editing core complex (RECC), is involved in the post- transcriptional editing of mitochondrial RNA, a process involving the addition and deletion of uridine (U) nucleotides in the pre-mRNA (PubMed:12820966, PubMed:19465686, PubMed:20362585). Specifically, catalyzes the addition of one U to single-stranded RNA with a preference for a 3'-terminal A or G and adds the number of Us specified by a guide RNA (gRNA) to precleaved double-stranded RNA editing substrates (PubMed:12820966, PubMed:19465686, PubMed:20362585, PubMed:16281058). Essential for the survival of the bloodstream form (PubMed:16281058). {ECO:0000269|PubMed:12820966, ECO:0000269|PubMed:16281058, ECO:0000269|PubMed:19465686, ECO:0000269|PubMed:20362585}. |
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| Catalytic activity: | |
Reaction=RNA(n) + UTP = diphosphate + RNA(n)-3'-uridine ribonucleotide; Xref=Rhea:RHEA:14785, Rhea:RHEA-COMP:11128, Rhea:RHEA-COMP:14648, ChEBI:CHEBI:33019, ChEBI:CHEBI:46398, ChEBI:CHEBI:83400, ChEBI:CHEBI:140627; EC=2.7.7.52; Evidence={ECO:0000269|PubMed:12820966, ECO:0000269|PubMed:16281058, ECO:0000269|PubMed:19465686, ECO:0000269|PubMed:20362585}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000305|PubMed:16281058}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000305|PubMed:16281058}; Note=Binds 1 Mg(2+) or Mn(2+) per subunit. {ECO:0000269|PubMed:16281058}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.2 uM for UTP (with 6(U) single-stranded RNA as substrate) {ECO:0000269|PubMed:19465686}; KM=3.4 uM for UTP (with double-stranded RNA as substrate) {ECO:0000269|PubMed:19465686}; KM=2.5 uM for UTP (with RNA with no U in 3' position as substrate) {ECO:0000269|PubMed:19465686}; KM=12.6 uM for UTP (with double-stranded RNA with one U in 3' position as substrate) {ECO:0000269|PubMed:19465686}; Note=kcat is 0.0007 min(-1) with UTP and 6(U) single-stranded RNA as substrates (PubMed:19465686). kcat is 0.002 min(-1) with UTP and double-stranded RNA as substrates (PubMed:19465686). kcat is 0.02 min(-1) with UTP and with RNA with no U in 3' position as substrates (PubMed:20362585). kcat is 0.2 min(-1) with UTP and double-stranded RNA with one U in 3' position as substrates (PubMed:20362585). {ECO:0000269|PubMed:19465686, ECO:0000269|PubMed:20362585}; |
| Subunit: | |
Component of the mitochondrial RNA editing core complex (RECC), also known as the editosome complex (PubMed:12820966, PubMed:12649499, PubMed:19465686, PubMed:20362585). Interacts (via middle domain) with MP81; the interaction enhances MP57 catalytic activity and probably recruits MP57 to the RECC complex (PubMed:12820966, PubMed:20362585). {ECO:0000269|PubMed:12649499, ECO:0000269|PubMed:12820966, ECO:0000269|PubMed:19465686, ECO:0000269|PubMed:20362585}. |
| Subcellular location: | |
Mitochondrion {ECO:0000269|PubMed:12649499, ECO:0000269|PubMed:12820966, ECO:0000269|PubMed:19465686}. |
| Disruption phenotype: | |
RNAi-mediated knockdown in the procyclic form causes growth arrest. {ECO:0000269|PubMed:20362585}. |
| Similarity: | |
Belongs to the DNA polymerase type-B-like family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.