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PDBsum entry 2b1u
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Metal binding protein
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PDB id
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2b1u
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Contents |
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* Residue conservation analysis
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DOI no:
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Structure
14:1029-1038
(2006)
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PubMed id:
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A structural and dynamic characterization of the EF-hand protein CLSP.
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E.Babini,
I.Bertini,
F.Capozzi,
E.Chirivino,
C.Luchinat.
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ABSTRACT
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The structure and dynamics of human calmodulin-like skin protein (CLSP) have
been characterized by NMR spectroscopy. The mobility of CLSP has been found to
be different for the N-terminal and C-terminal domains. The isolated domains
were also expressed and analyzed. The structure of the isolated C-terminal
domain is presented. The N-terminal domain is characterized by four stable
helices, which experience large fluctuations. This is shown to be due to
mutations in the hydrophobic core. The overall N-terminal domain behavior is
similar both in the full-length protein and in the isolated domain. By
exploiting the capability of Tb3+ bound to CLSP to induce partial orientation of
the molecule in a magnetic field, restricted motion of one domain with respect
to the other was proved. By using NMR, ITC, and ESI-MS, the calcium and
magnesium binding properties were investigated. Finally, CLSP is framed into the
evolutionary scheme of the calmodulin-like family.
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Selected figure(s)
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Figure 3.
Figure 3. Relaxation Parameters
^15N relaxation
parameters of full-length CLSP measured at 500 MHz and 25°C.
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Figure 4.
Figure 4. Comparison of CaM Structure and CLSP Model
Space-filling representation of the experimental CaM N-terminal
domain structure (PDB:1CFC) and of the CLSP N-terminal domain
model. The model was generated by the program MODELLER (6v2)
with the N-terminal domain of CaM (PDB: 1CFC) as template. (A),
(B), and (C) refer to different orientations of the protein
structures.
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The above figures are
reprinted
by permission from Cell Press:
Structure
(2006,
14,
1029-1038)
copyright 2006.
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Figures were
selected
by an automated process.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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W.Ohashi,
H.Hirota,
and
T.Yamazaki
(2011).
Solution structure and fluctuation of the Mg(2+)-bound form of calmodulin C-terminal domain.
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Protein Sci,
20,
690-701.
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PDB code:
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X.Huang,
M.Beullens,
J.Zhang,
Y.Zhou,
E.Nicolaescu,
B.Lesage,
Q.Hu,
J.Wu,
M.Bollen,
and
Y.Shi
(2009).
Structure and function of the two tandem WW domains of the pre-mRNA splicing factor FBP21 (formin-binding protein 21).
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J Biol Chem,
284,
25375-25387.
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PDB code:
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Y.Liu,
and
J.A.Cowan
(2009).
Iron-sulfur cluster biosynthesis: characterization of a molten globule domain in human NFU.
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Biochemistry,
48,
7512-7518.
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E.Johnson,
L.Bruschweiler-Li,
S.A.Showalter,
G.W.Vuister,
F.Zhang,
and
R.Brüschweiler
(2008).
Structure and dynamics of Ca2+-binding domain 1 of the Na+/Ca2+ exchanger in the presence and in the absence of Ca2+.
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J Mol Biol,
377,
945-955.
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O.Okhrimenko,
and
I.Jelesarov
(2008).
A survey of the year 2006 literature on applications of isothermal titration calorimetry.
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J Mol Recognit,
21,
1.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
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Links
