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UniProt functional annotation for P0AB83 | ||
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| UniProt code: P0AB83. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'- phosphate. {ECO:0000255|HAMAP-Rule:MF_00942, ECO:0000269|PubMed:2449657, ECO:0000269|PubMed:2669955, ECO:0000269|PubMed:6371006}. |
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| Catalytic activity: | |
Reaction=(DNA)-(deoxyribonucleoside 5'-phosphate)-(deoxyribose 5'- phosphate)-(deoxyribonucleoside 5'-phosphate) = (DNA)-3'- (deoxyribonucleoside 5'-phosphate)-(2,3-dehydro-2,3-deoxyribose 5'- phosphate) + H(+) + phospho-5'-(DNA); Xref=Rhea:RHEA:66592, Rhea:RHEA-COMP:13180, Rhea:RHEA-COMP:16897, Rhea:RHEA-COMP:17067, ChEBI:CHEBI:15378, ChEBI:CHEBI:136412, ChEBI:CHEBI:157695, ChEBI:CHEBI:167181; EC=4.2.99.18; Evidence={ECO:0000255|HAMAP- Rule:MF_00942, ECO:0000269|PubMed:2449657}; |
| Cofactor: | |
Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Evidence={ECO:0000255|HAMAP-Rule:MF_00942, ECO:0000269|PubMed:7664751, ECO:0000269|PubMed:8092678}; Note=Binds 1 [4Fe-4S] cluster. The cluster does not appear to play a role in catalysis, but is probably involved in DNA-binding and proper positioning of the enzyme along the DNA strand. {ECO:0000255|HAMAP- Rule:MF_00942, ECO:0000269|PubMed:7664751, ECO:0000269|PubMed:8092678}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:6371006}. |
| Similarity: | |
Belongs to the Nth/MutY family. {ECO:0000255|HAMAP- Rule:MF_00942}. |
Annotations taken from UniProtKB at the EBI.