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PDBsum entry 2yep
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protein ligands Protein-protein interface(s) links
Transferase PDB id
2yep

 

 

 

 

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Contents
Protein chains
173 a.a.
213 a.a.
197 a.a.
Ligands
GLU ×2
ACT ×2
Waters ×419
PDB id:
2yep
Name: Transferase
Title: Structure of an n-terminal nucleophile (ntn) hydrolase, oat2, in complex with glutamate
Structure: Glutamate n-acetyltransferase 2 alpha chain. Chain: a, c, e, g. Synonym: ornithine acetyltransferase 2 alpha chain, ornithine transacetylase 2 alpha chain, oatase 2 alpha chain. Engineered: yes. Glutamate n-acetyltransferase 2 beta chain. Chain: b, d, h. Synonym: ornithine acetyltransferase 2 beta chain, ornithine transacetylase 2 beta chain, oatase 2 beta chain.
Source: Streptomyces clavuligerus. Organism_taxid: 1901. Atcc: 3585. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
2.70Å     R-factor:   0.218     R-free:   0.236
Authors: R.Chowdhury,A.Iqbal,I.J.Clifton,C.J.Schofield
Key ref: A.Iqbal et al. (2011). Structural and biochemical analyses reveal how ornithine acetyl transferase binds acidic and basic amino acid substrates. Org Biomol Chem, 9, 6219-6225. PubMed id: 21796301
Date:
29-Mar-11     Release date:   07-Sep-11    
Supersedes: 2w4n
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P0DJQ5  (GNAT2_STRCL) -  Glutamate N-acetyltransferase 2 from Streptomyces clavuligerus
Seq:
Struc: 		393 a.a.
173 a.a.
Protein chains
Pfam   ArchSchema ?
P0DJQ5  (GNAT2_STRCL) -  Glutamate N-acetyltransferase 2 from Streptomyces clavuligerus
Seq:
Struc: 		393 a.a.
213 a.a.*
Protein chains
Pfam   ArchSchema ?
P0DJQ5  (GNAT2_STRCL) -  Glutamate N-acetyltransferase 2 from Streptomyces clavuligerus
Seq:
Struc: 		393 a.a.
197 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: Chains A, B, C, D, E, F, G, H: E.C.2.3.1.35  - glutamate N-acetyltransferase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: N2-acetyl-L-ornithine + L-glutamate = N-acetyl-L-glutamate + L-ornithine
N(2)-acetyl-L-ornithine
Bound ligand (Het Group name = GLU)
matches with 90.00% similarity 		+ L-glutamate
 = N-acetyl-L-glutamate
 + L-ornithine
Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 

 
    reference    
 
 
Org Biomol Chem 9:6219-6225 (2011)
PubMed id: 21796301  
 
 
Structural and biochemical analyses reveal how ornithine acetyl transferase binds acidic and basic amino acid substrates.
A.Iqbal, I.J.Clifton, R.Chowdhury, D.Ivison, C.Domene, C.J.Schofield.
 
  ABSTRACT  
 
No abstract given.

 

 

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