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PDBsum entry 2xmc

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protein ligands metals links
Hydrolase PDB id
2xmc
Jmol
Contents
Protein chain
527 a.a. *
Ligands
UNX-UNX ×2
UNX ×9
UNX-UNX-UNX-UNX
SO4
NAG-NAG-FUC ×2
NAG-FUC
NAG ×3
Metals
_CL ×5
__F
__K
Waters ×229
* Residue conservation analysis
PDB id:
2xmc
Name: Hydrolase
Title: G117h mutant of human butyrylcholinesterase in complex with fluoride anion
Structure: Cholinesterase. Chain: a. Fragment: residues 29-557. Synonym: acylcholine acylhydrolase, choline esterase ii, butyrylcholinesterase, pseudocholinesterase. Engineered: yes. Mutation: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: cricetulus griseus. Expression_system_taxid: 10029. Expression_system_cell_line: cho k1
Resolution:
2.40Å     R-factor:   0.193     R-free:   0.252
Authors: F.Nachon,E.Carletti,M.Wandhammer,Y.Nicolet,L.M.Schopfer,P.Ma O.Lockridge
Key ref: F.Nachon et al. (2011). X-ray crystallographic snapshots of reaction intermediates in the G117H mutant of human butyrylcholinesterase, a nerve agent target engineered into a catalytic bioscavenger. Biochem J, 434, 73-82. PubMed id: 21091433
Date:
27-Jul-10     Release date:   01-Dec-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P06276  (CHLE_HUMAN) -  Cholinesterase
Seq:
Struc:
 
Seq:
Struc:
602 a.a.
527 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 5 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.3.1.1.8  - Cholinesterase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: An acylcholine + H2O = choline + a carboxylate
acylcholine
+ H(2)O
= choline
+ carboxylate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   7 terms 
  Biological process     response to drug   13 terms 
  Biochemical function     catalytic activity     9 terms  

 

 
    reference    
 
 
Biochem J 434:73-82 (2011)
PubMed id: 21091433  
 
 
X-ray crystallographic snapshots of reaction intermediates in the G117H mutant of human butyrylcholinesterase, a nerve agent target engineered into a catalytic bioscavenger.
F.Nachon, E.Carletti, M.Wandhammer, Y.Nicolet, L.M.Schopfer, P.Masson, O.Lockridge.
 
  ABSTRACT  
 
No abstract given.