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PDBsum entry 2xe8

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protein ligands links
Transferase PDB id
2xe8
Jmol
Contents
Protein chain
413 a.a. *
Ligands
3PG
ACP
Waters ×140
* Residue conservation analysis
PDB id:
2xe8
Name: Transferase
Title: The complete reaction cycle of human phosphoglycerate kinase: the open ternary complex with 3pg and amp-pnp
Structure: Phosphoglycerate kinase 1. Chain: a. Synonym: primer recognition protein 2, prp 2, cell migration-inducing gene 10 protein. Engineered: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Resolution:
1.79Å     R-factor:   0.200     R-free:   0.242
Authors: M.J.Cliff,N.J.Baxter,G.M.Blackburn,A.Merli,M.Vas,J.P.Waltho, M.W.Bowler
Key ref: L.Zerrad et al. (2011). A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinase. J Biol Chem, 286, 14040-14048. PubMed id: 21349853 DOI: 10.1074/jbc.M110.206813
Date:
11-May-10     Release date:   19-Jan-11    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00558  (PGK1_HUMAN) -  Phosphoglycerate kinase 1
Seq:
Struc:
417 a.a.
413 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.2.7.2.3  - Phosphoglycerate kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Calvin Cycle (carbon fixation stages)
      Reaction: ATP + 3-phospho-D-glycerate = ADP + 3-phospho-D-glyceroyl phosphate
ATP
+
3-phospho-D-glycerate
Bound ligand (Het Group name = 3PG)
corresponds exactly
=
ADP
Bound ligand (Het Group name = ACP)
matches with 81.25% similarity
+ 3-phospho-D-glyceroyl phosphate
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   3 terms 
  Biological process     small molecule metabolic process   7 terms 
  Biochemical function     nucleotide binding     6 terms  

 

 
    reference    
 
 
DOI no: 10.1074/jbc.M110.206813 J Biol Chem 286:14040-14048 (2011)
PubMed id: 21349853  
 
 
A spring-loaded release mechanism regulates domain movement and catalysis in phosphoglycerate kinase.
L.Zerrad, A.Merli, G.F.Schröder, A.Varga, ..Gráczer, P.Pernot, A.Round, M.Vas, M.W.Bowler.
 
  ABSTRACT  
 
No abstract given.