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PDBsum entry 2vsh

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protein ligands metals Protein-protein interface(s) links
Transferase PDB id
2vsh
Jmol
Contents
Protein chains
223 a.a. *
Ligands
PEG ×3
1PE
PG4
P6G
Metals
_CA ×2
Waters ×240
* Residue conservation analysis
PDB id:
2vsh
Name: Transferase
Title: Synthesis of cdp-activated ribitol for teichoic acid precursors in streptococcus pneumoniae
Structure: 2-c-methyl-d-erythritol 4-phosphate cytidylyltransferase. Chain: a, b. Synonym: tari. Ec: 2.7.7.60
Source: Streptococcus pneumoniae. Organism_taxid: 1313. Strain: r36a. Atcc: 12214
Resolution:
2.00Å     R-factor:   0.199     R-free:   0.254
Authors: S.Baur,J.Marles-Wright,S.Buckenmaier,R.J.Lewis,W.Vollmer
Key ref: S.Baur et al. (2009). Synthesis of CDP-activated ribitol for teichoic acid precursors in Streptococcus pneumoniae. J Bacteriol, 191, 1200-1210. PubMed id: 19074383
Date:
23-Apr-08     Release date:   30-Dec-08    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
A5MSS9  (A5MSS9_STRPN) -  2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase
Seq:
Struc:
235 a.a.
223 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: E.C.2.7.7.60  - 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
CTP
+ 2-C-methyl-D-erythritol 4-phosphate
= diphosphate
+ 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol
      Cofactor: Manganese or magnesium
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     isoprenoid biosynthetic process   3 terms 
  Biochemical function     catalytic activity     4 terms  

 

 
    reference    
 
 
J Bacteriol 191:1200-1210 (2009)
PubMed id: 19074383  
 
 
Synthesis of CDP-activated ribitol for teichoic acid precursors in Streptococcus pneumoniae.
S.Baur, J.Marles-Wright, S.Buckenmaier, R.J.Lewis, W.Vollmer.
 
  ABSTRACT  
 
Streptococcus pneumoniae has unusually complex cell wall teichoic acid and lipoteichoic acid, both of which contain a ribitol phosphate moiety. The lic region of the pneumococcal genome contains genes for the uptake and activation of choline, the attachment of phosphorylcholine to teichoic acid precursors, and the transport of these precursors across the cytoplasmic membrane. The role of two other, so far uncharacterized, genes, spr1148 and spr1149, in the lic region was determined. TarJ (spr1148) encodes an NADPH-dependent alcohol dehydrogenase for the synthesis of ribitol 5-phosphate from ribulose 5-phosphate. TarI (spr1149) encodes a cytidylyl transferase for the synthesis of cytidine 5'-diphosphate (CDP)-ribitol from ribitol 5-phosphate and cytidine 5'-triphosphate. We also present the crystal structure of TarI with and without bound CDP, and the structures present a rationale for the substrate specificity of this key enzyme. No transformants were obtained with insertion plasmids designed to interrupt the tarIJ genes, indicating that their function could be essential for cell growth. CDP-activated ribitol is a precursor for the synthesis of pneumococcal teichoic acids and some of the capsular polysaccharides. Thus, all eight genes in the lic region have a role in teichoic acid synthesis.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
22522421 T.Roscioli, E.J.Kamsteeg, K.Buysse, I.Maystadt, J.van Reeuwijk, C.van den Elzen, E.van Beusekom, M.Riemersma, R.Pfundt, L.E.Vissers, M.Schraders, U.Altunoglu, M.F.Buckley, H.G.Brunner, B.Grisart, H.Zhou, J.A.Veltman, C.Gilissen, G.M.Mancini, P.Delrée, M.A.Willemsen, D.P.Ramadža, D.Chitayat, C.Bennett, E.Sheridan, E.A.Peeters, G.M.Tan-Sindhunata, C.E.de Die-Smulders, K.Devriendt, H.Kayserili, O.A.El-Hashash, D.L.Stemple, D.J.Lefeber, Y.Y.Lin, and H.van Bokhoven (2012).
Mutations in ISPD cause Walker-Warburg syndrome and defective glycosylation of α-dystroglycan.
  Nat Genet, 44, 581-585.  
21543842 C.Björkelid, T.Bergfors, L.M.Henriksson, A.L.Stern, T.Unge, S.L.Mowbray, and T.A.Jones (2011).
Structural and functional studies of mycobacterial IspD enzymes.
  Acta Crystallogr D Biol Crystallogr, 67, 403-414.
PDB codes: 2xwl 2xwm 2xwn
19916033 C.Obiol-Pardo, A.Cordero, J.Rubio-Martinez, and S.Imperial (2010).
Homology modeling of Mycobacterium tuberculosis 2C-methyl-D-erythritol-4-phosphate cytidylyltransferase, the third enzyme in the MEP pathway for isoprenoid biosynthesis.
  J Mol Model, 16, 1061-1073.  
20180114 V.Monedero, G.Pérez-Martínez, and M.J.Yebra (2010).
Perspectives of engineering lactic acid bacteria for biotechnological polyol production.
  Appl Microbiol Biotechnol, 86, 1003-1015.  
19737355 A.Eberhardt, L.J.Wu, J.Errington, W.Vollmer, and J.W.Veening (2009).
Cellular localization of choline-utilization proteins in Streptococcus pneumoniae using novel fluorescent reporter systems.
  Mol Microbiol, 74, 395-408.  
19464183 O.Rahman, L.G.Dover, and I.C.Sutcliffe (2009).
Lipoteichoic acid biosynthesis: two steps forwards, one step sideways?
  Trends Microbiol, 17, 219-225.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.